Diversity and authentication of accessions revealed by complete plastid genome and rDNA sequences.

Mitochondrial DNA B Resour

Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.

Published: April 2021


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Article Abstract

Complete plastid genome (plastome) and ribosomal DNA (rDNA) sequences of three accessions (two and one ) were newly assembled using Illumina whole-genome sequences. Nakai and var. tozawai, described as different varieties, have identical plastomes and rDNA sequences. The plastomes are 155,957 bp and 156,005 bp and the 45S rDNA transcription unit sizes are 5809 bp and 5811 bp in and , respectively. The 5S rDNA transcription unit is an identical 121 bp in three accessions. We developed three DNA markers to authenticate and based on plastome diversity. Phylogenomic analysis revealed that the species classified as two clades and , , and are the most closely related species in clade 1.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079122PMC
http://dx.doi.org/10.1080/23802359.2021.1911712DOI Listing

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