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In this study, we investigated the incidence of Cronobacter spp. in seafood collected from retail fish markets of Mumbai, India. A total of 50 samples comprising fresh finfish (n = 32), shellfish (n = 6), dried fish (n = 9) and water (n = 3) were analyzed for Cronobacter spp. by selective enrichment, isolation and biochemical tests. Of 145 isolates presumptively identified as Cronobacter spp. by biochemical tests, 37 were confirmed as Cronobacter spp. by Polymerase Chain Reaction (PCR) specific to the internal transcribed spacer (ITS) regions. Based on the partial ITS gene sequence analysis, 35 isolates were identified as Cronobacter malonaticus and two as Cronobacter sakazakii. The highest incidence of Cronobacter spp. was in dried fish (55.6%), followed by shellfish (33.3%). The virulence gene ompA was detected in two Cronobacter sakazakii isolates. This is the first report of the incidence of Cronobacter spp. in fresh and dried seafood from India, which highlights the need to focus on this emerging pathogen in tropical seafood.
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http://dx.doi.org/10.1007/s00284-021-02447-3 | DOI Listing |
Appl Environ Microbiol
September 2025
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.
spp. is an important foodborne pathogen that causes serious neonatal infections. The prevalence of the colistin resistance gene in spp.
View Article and Find Full Text PDFFoods
July 2025
Department of Agricultural and Food Sciences, University of Bologna, Piazza Goidanich 60, 47521 Cesena, Italy.
The consumption of fresh fruit and vegetables is essential for a healthy diet as they contain a diverse composition of vitamins, minerals, fibre, and bioactive compounds. However, cross-contamination during harvest and post-harvest poses a high risk of microbial contamination. Therefore, handling fruit and vegetables during processing and contact with wet equipment and utensil surfaces is an ideal environment for microbial contamination and foodborne illness.
View Article and Find Full Text PDFBull Exp Biol Med
May 2025
Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russia.
Contamination of dry foods with the Cronobacter genus bacteria poses a significant challenge for ensuring microbiological safety of food products for infants and children, because these pathogens can cause severe infections in infants. We studied Cronobacter strains isolated from samples of dry infant food products sold in the Russian Federation, as well as components for their production. It was found that all the isolated strains belonged to the most epidemiologically significant Cronobacter sakazakii species, and the presence of at least four genes characterizing different virulence mechanisms was confirmed for all strains.
View Article and Find Full Text PDFJ Food Prot
August 2025
Department of Food Science, Cornell University, Ithaca, NY, USA. Electronic address:
Cronobacter spp. contamination in powdered infant formula (PIF) can cause infections in high-risk infants. Public health guidelines for caregivers of high-risk infants advise reconstitution of PIF using water heated to at least 70 °C (158 °F) for microbial inactivation.
View Article and Find Full Text PDFJ Food Prot
July 2025
Jianyuan Science & Technology Co. Ltd., Shanghai 200136, China; Guangdong Maoming Agriculture & Forestry Technical College, Maoming 525024, China. Electronic address:
Foodborne bacteria such as Cronobacter are opportunistic and have been connected to potentially fatal infections. The current work created a fast and sensitive RNA targeting amplification and detection system for Cronobacter spp., including enrichment, RNA isolation, and detection by real-time RNA isothermal amplification, capable of detecting viable Cronobacter spp.
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