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Article Abstract
Two-photon (2-P) all-optical approaches combine 2-P calcium imaging and 2-P optogenetic modulations. Here, firstly, we combined juxtacellular recordings and GCaMP6f-based 2-P calcium imaging in mouse visual cortex to tune our detection algorithm towards a 100% specific identification of action potential-related calcium transients. Secondly, we minimized photostimulation artifacts by using extended-wavelength-spectrum laser sources for optogenetic stimulation. We achieved artifact-free all-optical experiments performing optogenetic stimulation from 1100 nm to 1300 nm. Thirdly, we determined the spectral range for maximizing efficacy until 1300 nm. The rate of evoked transients in GCaMP6f/C1V1-co-expressing cortical neurons peaked already at 1100 nm. By refining spike detection and defining 1100 nm as the optimal wavelength for artifact-free and effective GCaMP6f/C1V1-based all-optical physiology, we increased the translational value of these approaches, e.g., for the development of network-based therapies.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921810 | PMC |
| http://dx.doi.org/10.1016/j.isci.2021.102184 | DOI Listing |
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