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Organochlorine pesticides (OCPs) have received much attention due to their toxicity. Reliable methods to monitor their residues in the environment are needed. Here, magnetic polyamidoamine dendrimers were prepared by co-precipitation, Michael addition, and amidation. The magnetic polyamidoamine dendrimers demonstrated good adsorption ability for OCPs-this feature was utilized to construct a sensitive tool for monitoring OCPs in water samples. The proposed method provided remarkable linearity from 0.1 to 500 μg/L and satisfactory limits of detection from 0.012 to 0.029 μg/L. The spiked recoveries of the four target analytes were 91.8%-103.5% with relative standard deviations less than 4.5%. The magnetic materials had good reusability. The results indicated that the resulting method was an efficient, easy, rapid, economical, and eco-friendly tool for monitoring OCPs in aqueous samples.
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http://dx.doi.org/10.1016/j.jes.2020.09.005 | DOI Listing |
Biomacromolecules
August 2025
Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, via Mancinelli 7, Milano 20131, Italy.
This work presents a novel strategy for enhancing the gene delivery capabilities of low-generation poly(amidoamine) (PAMAM) dendrimers by employing a fluorinated, redox-responsive cross-linker. The synthesized cross-linker, featuring both disulfide bonds and trifluoromethyl groups, facilitates efficient "click" Michael addition with PAMAM G2, producing cross-linked polymers with strong F nuclear magnetic resonance signals and favorable relaxation properties, enabling their use as F nuclear magnetic resonance tracers. The resulting fluorinated PAMAM G2 polymers also demonstrated excellent plasmid DNA complexation, glutathione-triggered degradation, and notably enhanced transfection efficiency, especially in cancerous HeLa cells notoriously associated with a reducing environment, while maintaining low cytotoxicity.
View Article and Find Full Text PDFLangmuir
August 2025
School of Chemistry and Chemical Engineering, Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University, Nanjing, Jiangsu 211189, P. R. China.
This study aims to enhance the signal-to-noise ratio (SNR) in chemiluminescence immunoassays (CLIA) by increasing the protein immobilization capacity on magnetic bead surfaces. Proteins are macromolecules with three-dimensional conformations where merely increasing the density of surface functional groups on magnetic beads is insufficient to substantially enhance protein immobilization. To address this limitation, we grafted polyamidoamine (PAMAM) dendrimers with three-dimensional architectures onto magnetic beads, simultaneously augmenting the functional group density and expanding the spatial distribution to improve protein loading capacity.
View Article and Find Full Text PDFThe successful application of enzymes in industries encounters challenges related to high costs, stability, and reuse. In this study, β-glucosidase (BGL) was immobilized via a covalent method after the synthesis of different generations (G) of polyamidoamine (PAMAM) dendrimers modified magnetic spent coffee grounds (SCGs). With the increase in PAMAM generation, BGL immobilization (163.
View Article and Find Full Text PDFAnal Sci
August 2025
Department of Chemistry, Università degli Studi di Milano, via Golgi 19, 20133, Milan, Italy.
Polyamidoamines obtained through the polyaddition of natural alpha-aminoacids to N,N'-methylenebisacrylamide were shown to be remarkably active flame retardants for cotton. The presence of a disulfide linkage together with phosphorus atoms was previously chosen as desirable structural requirements and added to the polymer chain for their flame retardants properties. The conjugation of cystamine with diethyl vinyl phosphonate to obtain the desired monomer is accompanied by the formation of a by-product that must be identified and characterized to optimize the reaction.
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