Covalent Immobilization of β-Glucosidase on Magnetic Spent Coffee Grounds Modified With PAMAM Dendrimers for Enhanced Glucose Tolerance.

The successful application of enzymes in industries encounters challenges related to high costs, stability, and reuse. In this study, β-glucosidase (BGL) was immobilized via a covalent method after the synthesis of different generations (G) of polyamidoamine (PAMAM) dendrimers modified magnetic spent coffee grounds (SCGs). With the increase in PAMAM generation, BGL immobilization (163.

β-Carotene Production by Oleaginous Yeasts in a Pilot Plant Fermenter: Yield Standardization and Process Scale-Up.

This study reports selective β-carotene pigment production by an oleaginous yeast, IIPL32 (MTCC 25056), in a 500 L fermenter working scale with a practically feasible approach for microbial pigment production scale-up from small (500 mL) to pilot scale (500 L) submerged fermenters using medium rheology and fermenter hydrodynamics. The pigment production was initially standardized by optimizing the carbon and nitrogen load of the fermentation medium. The pigment was purified using a solvent extraction process followed by thin-layer chromatography and analyzed by UV-visible spectrophotometry, high-pressure liquid chromatography, and Fourier-transform infrared spectroscopy, suggesting the dominant presence of β-carotene.

Anaerobic conversion of de-oiled yeast biomass fractionation waste to biomethane and biohydrogen for resource efficiency in biorefineries.

High-value intracellular bio-compounds are extracted from microbial biomass through cell fractionation processes, which generate discharge streams. These discharges are rich in organic carbon and nitrogen that are derived from the soluble and insoluble protein and carbohydrate polymers. The present study investigated the anaerobic conversion of such a tertiary waste stream generated during the production of glucan-chitin complex through fractionation of de-oiled yeast biomass (a type of spent microbial biomass, which is the solid leftover residue of yeast lipid production process).

Magnetic β-glucosidase-inorganic hybrid nanoflowers: Highly active and recyclable nano-biocatalyst with increased glucose tolerance for cellobiose hydrolysis.

The high surface area, increased enzyme activity, active site accessibility and improved stability of the enzyme-inorganic hybrid nanoflowers have garnered significant attention in recent years. In this study, we have synthesized magnetic β-glucosidase-Cu(PO) nanoflowers (BGL@MNF) using the co-precipitation method. In the presence of magnetic nanoparticles, the catalytic efficiency (K/K) of β-glucosidase is 56.

Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste.

The challenges of pollution and agro-industrial waste management have led to the development of bioconversion techniques to transform these wastes into valuable products. This has increased the focus on the sustainable and cost-efficient production of biosurfactants from agro-industrial waste. Hence, the present study investigates the production of sophorolipid biosurfactants using the yeast strain IIPL32 under submerged fermentation, employing sugarcane bagasse hydrolysate-a renewable, low-cost agro-industrial waste as the feedstock.

Effects of advanced oxidation process on biological treatment of spent fermentation broth.

The present study aimed to establish the feasibility of the wastewater treatment process generated from an oleaginous fermentation plant. Treatment of spent fermentation broth (SFB) poses significant environmental challenges due to its high organic load, recalcitrant compounds, and potential toxicity. The synergistic effects of combining ozone-based advanced oxidation process (O-AOP) with biological treatment for the efficient degradation of pollutants in spent fermentation broth.

Bioproduction of yeast single cell oil with acute oral toxicity study intended for edible oil application.

Human nutrition and health rely on edible oils. Global demand for edible oils is expanding, necessitating the discovery of new natural oil sources subjected to adequate quality and safety evaluation. However, in contrast to other agricultural products, India's edible oil supply is surprisingly dependent on imports.

Enhanced remediation of polyaromatic hydrocarbon using agro-industrial waste for biofuel production and environmental pollution mitigation.

In the present study, attention has been paid to the development of economically feasible strategies for enhanced remediation of anthracene and its conversion into biofuels. The strategies developed (B1, B2, B3, and B4) include bagasse and lipid-producing strain Rhodotorula mucilagenosa IIPL32 synthesizing surface active metabolites. The results indicate the highest production of surface-active metabolites in strategies B2, B3, and B4 along with a maximum biodegradation rate.

Time-resolved transcriptomic profile of oleaginous yeast Rhodotorula mucilaginosa during lipid and carotenoids accumulation on glycerol.

The study reports the exploration of the transcriptome landscape of the red oleaginous yeast Rhodotorula mucilaginosa IIPL32 coinciding with the fermentation kinetics of the yeast cultivated in a two-stage fermentation process to exploit the time-series approach to get the complete transcripts picture and reveal the persuasive genes for fatty acid and terpenoid synthesis. The finding displayed the molecular drivers with more than 2-fold upregulation in the nitrogen-limited stage than in the nitrogen-excess stage. The rate-limiting diphosphomevalonate decarboxylase, acetylCoA-citrate lyase, and acetyl-CoA C-acetyltransferase were significant in controlling the metabolic flux in the synthesis of reduced compounds, and acetoacetyl-CoA synthase, 3-ketoacyl-acyl carrier-protein reductase, and β-subunit enoyl reductase catalyze the key starting steps of lipids or terpenoid synthesis.

An insight into omics analysis and metabolic pathway engineering of lignin-degrading enzymes for enhanced lignin valorization.

Lignin, a highly heterogeneous polymer of lignocellulosic biomass, is intricately associated with cellulose and hemicellulose, responsible for its strength and rigidity. Lignin decomposition is carried out through certain enzymes derived from microorganisms to promote the hydrolysis of lignin. Analyzing multi-omics data helps to emphasize the probable value of fungal-produced enzymes to degrade the lignocellulosic material, which provides them an advantage in their ecological niches.

Water footprint and wastewater quality assessment of yeast single cell oil production: Gate to gate approach for industrial water sustainability.

Effective water resource utilization and sustainability for industrial operations is a growing concern. With increased industrial water demand, abstraction and water quality changes are rising. In India, distilleries generate more than 40.

Efficient valorization of feather waste by Bacillus cereus IIPK35 for concomitant production of antioxidant keratin hydrolysate and milk-clotting metallo-serine keratinase.

Keratinase production by Bacillus cereus IIPK35 was investigated under solid-state fermentation (SSF) and the maximum titer of 648.28 U/gds was revealed. Feather hydrolysates obtained from SSF exhibited paramount antioxidant properties in ABTS [2,2'-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid], FRAP [Ferric ion reducing antioxidant power], and DPPH [2,2,-Diphenyl-1-picrylhydrazyl] assay.

Remediation and detoxification of oil contaminated marine intertidal sites through lipopeptide assisted washing strategy: An experimental and kinetic validation approach.

This paper presents a tightly coupled experimental and kinetic approach for efficient remediation of oil spill from contaminated marine intertidal zone surface through a methodical strategy that deals with biosurfactant mediated washing strategy. The study deals with production, optimization and characterization of lipopeptide biosurfactant from Bacillus subtilis T1 and its application in remediation of oil contaminants from mimic model system of various marine intertidal zone i.e.

A machine learning-based approach to determine infection status in recipients of BBV152 (Covaxin) whole-virion inactivated SARS-CoV-2 vaccine for serological surveys.

Data science has been an invaluable part of the COVID-19 pandemic response with multiple applications, ranging from tracking viral evolution to understanding the vaccine effectiveness. Asymptomatic breakthrough infections have been a major problem in assessing vaccine effectiveness in populations globally. Serological discrimination of vaccine response from infection has so far been limited to Spike protein vaccines since whole virion vaccines generate antibodies against all the viral proteins.

Variations in the SDN Loop of Class A Beta-Lactamases: A Study of the Molecular Mechanism of BlaC () to Alter the Stability and Catalytic Activity Towards Antibiotic Resistance of MBIs.

The emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis calls for an immediate search for novel treatment strategies. Recently, BlaC, the principal beta-lactamase of , was recognized as a potential therapeutic target. BlaC belongs to Ambler class A, which is generally susceptible to the beta-lactamase inhibitors currently used in clinics: tazobactam, sulbactam, and clavulanate.

Pyrolysis of de-oiled yeast biomass of Rhodotorula mucilaginosa IIPL32: Kinetics and thermodynamic parameters using thermogravimetric analysis.

The increasing demand for natural resources has highlighted the need to search for unutilized carbon resource that satisfy the demand and pose a minor threat to the environment. Yeast is a microbe with large industrial applications, and the biomass leftover after fermentation needs utilization for achieving increased efficiency. De-oiled yeast biomass (DYB), the residue after yeast lipid extraction, has not yet been evaluated for its potential application in the pyrolysis process.

Characterization of the de-oiled yeast biomass for plausible value mapping in a biorefinery perspective.

Oleaginous yeast fermentation process has gained attention for yeast single cell oil production. However, after lipid extraction, the leftover de-oiled yeast biomass has not been investigated in detail for its suitability for thermochemical conversion. To understand the structural and morphological changes, the comparative characterization of yeast and de-oiled yeast biomass before and post lipid extraction is necessary.

Insights from a Pan India Sero-Epidemiological survey (Phenome-India Cohort) for SARS-CoV2.

To understand the spread of SARS-CoV2, in August and September 2020, the Council of Scientific and Industrial Research (India) conducted a serosurvey across its constituent laboratories and centers across India. Of 10,427 volunteers, 1058 (10.14%) tested positive for SARS-CoV2 anti-nucleocapsid (anti-NC) antibodies, 95% of which had surrogate neutralization activity.

Connecting the dots: Advances in modern metabolomics and its application in yeast system.

History of metabolism originates with yeast making bread. In fact, study based on "Yeast" was so crucial in the development of the field of biochemistry that the word "enzyme" is derived from the Greek word meaning leavened (yeast). Yeast has always been a point of interest as a eukaryotic model system to demonstrate the metabolites and their function.

Scale-up strategy for yeast single cell oil production for Rhodotorula mucilagenosa IIPL32 from corn cob derived pentosan.

This work was aimed to strategically scale-up the yeast lipid production process using Reynolds number as a standard rheological parameter from 50 mL to 50 L scale. Oleaginous yeast Rhodotorula mucilaginosa IIPL32 was cultivated in xylose rich corncob hydrolysate. The fermentation process for growth and maturation was operated in fed-batch with two different C/N ratios of 40 and 60.

Effect of utilization of crude glycerol as substrate on fatty acid composition of an oleaginous yeast Rhodotorula mucilagenosa IIPL32: Assessment of nutritional indices.

This work studied the use of crude glycerol obtained from biodiesel industry as substrate to generate yeast lipid from Rhodotorula mucilagenosa IIPL32 MTCC 25056. Crude glycerol is a low value by product obtained from biodiesel industry. Rhodotorula mucilagenosa IIPL32 MTCC 25056 was evaluated for its potential to produce lipid using crude glycerol as sole source of carbon.