Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). The test has two steps: 1) heat saliva with a stabilization solution, and 2) detect virus by incubating with a primer/enzyme mix. After incubation, saliva samples containing the SARS-CoV-2 genome turn bright yellow. Because this test is pH dependent, it can react falsely to some naturally acidic saliva samples. We report unique saliva stabilization protocols that rendered 295 healthy saliva samples compatible with the test, producing zero false positives. We also evaluated the test on 278 saliva samples from individuals who were infected with SARS-CoV-2 but had no symptoms at the time of saliva collection, and from 54 matched pairs of saliva and anterior nasal samples from infected individuals. The Saliva TwoStep test described herein identified infections with 94% sensitivity and >99% specificity in individuals with sub-clinical (asymptomatic or pre-symptomatic) infections.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7899473 | PMC |
| http://dx.doi.org/10.1101/2020.07.16.20150250 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of potential inflammation markers for outgrowth of cow's milk allergy.
PLoS One
September 2025
Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
Immunoglobulin E (IgE)-mediated cow's milk allergy (CMA) is an immune-mediated reaction to cow's milk (CM). Non-IgE-mediated CMA resolves in most children in the first years of life, whereas IgE-mediated CMA outgrowth is often later or not at all. The exact mechanisms underlying resolution of IgE-mediated CMA are not fully understood.
View Article and Find Full Text PDFBreath saliva for lung cancer detection with dogs.
ERJ Open Res
September 2025
School of Psychology, University of Waikato, Hamilton, New Zealand.
Background: While some research shows that dogs are able to detect lung cancer at above-chance levels using breath samples, the relative utility of other sample types has not been established. We evaluated the comparative utility of human breath and saliva samples for lung cancer detection using dogs.
Methods: Seven dogs assessed breath and saliva samples from 154 patients attending a general respiratory clinic.
Influence of Tea, Coffee, and Turmeric Solutions on the Color Stability of Denture Base Acrylic Resins and Silicone Soft Lining Material: An In Vitro Study.
Cureus
August 2025
Prosthodontics, Kerala University of Health Sciences, Thrissur, IND.
Background and objectives With the continuous presence of microflora, saliva, and frequent intake of coloured food, the colour stability of any aesthetic material may become compromised. Hence, the present study was conducted to evaluate the influence of tea, coffee, and turmeric solutions on the colour stability of commercially available heat-cured and autopolymerizing denture base acrylic resins as well as a soft lining material. Methods Twenty-four rectangular samples measuring 20 mm × 15 mm × 2 mm were prepared for each type of test material.
View Article and Find Full Text PDFDetermination of alcohol concentration in a single drop blood obtained via fingertip using gas chromatography/mass spectrometry coupled with solid-phase microextraction.
Leg Med (Tokyo)
September 2025
Department of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
This study investigated headspace solid-phase microextraction (HS-SPME)-gas chromatography (GS)/mass spectrometry as a low-complexity method for accurate measurement of blood alcohol concentration (BAC) changes in humans over time following alcohol consumption. The aim was to develop an analytical method that would require as small blood samples as possible-smaller than that required for the conventional method-thereby reducing the burden on the subject. Polyethylene glycol (PEG) was used as the fiber material for SPME, and a DB-WAX capillary column was used for GC.
View Article and Find Full Text PDFVirtual Reality Distraction vs Nitrous Oxide for Reducing Anxiety and Injection Pain.
Anesth Prog
September 2025
Objective: The purpose of this study was to compare nitrous oxide (N2O) vs virtual reality (VR) as methods for reducing pain and anxiety during a dental injection. The primary objectives were to assess acute changes in stress responses by comparing salivary cortisol levels between the 2 groups and differences in injection pain scores.
Methods: A total of 132 female subjects serving as their own control received maxillary lateral incisor infiltration injections with the use of either N2O or a VR headset during separate appointments spaced at least 2 weeks apart.