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Freshwater planarians are free-living flatworms known for their regenerative ability. Being easily cultured under laboratory conditions, they are recognized test model organisms in regeneration, developmental biology, and neuropharmacological research. Also, they have been recently employed in toxicity testing where they displayed an array of sensitive and reliable responses to environmental stressors. Here, we outline simple and easy-to-follow protocols to evaluate effects of environmental contaminants and other stressors on survival, behavior (feeding and locomotor activity), and regeneration of freshwater planarians. These endpoints are comparable with responses of well-established ecotoxicological model species.
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http://dx.doi.org/10.1007/978-1-0716-1091-6_11 | DOI Listing |
Environ Res
September 2025
School of Geography, Earth & Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom. Electronic address:
Human activities have introduced a wide range of contaminants into aquatic ecosystems, posing substantial ecological and health risks. Robust bioindicators are essential for accurately predicting these impacts. Since the early 1980s, planarians-freshwater flatworms known for their remarkable regenerative ability and neurologically relevant system-have been used in ecotoxicology, witnessing renewed scientific interest post-2010.
View Article and Find Full Text PDFEcotoxicology
August 2025
Instituto Federal de Educação Ciência e Tecnologia Goiano - Campus Campos Belos, Research Group: Laboratório de Conservação de Agroecossistemas e Ecotoxicologia - CAE Group, Campos Belos, Goiás, Brazil.
This study evaluates the acute and chronic toxicity of imidacloprid (IMI), a neonicotinoid insecticide (NNI) commonly used worldwide, on the non-target planarian Girardia tigrina. The research is motivated by the frequent detection of IMI in aquatic ecosystems near agricultural areas, raising concerns about its adverse effects on non-target organisms. Acute toxicity was assessed by exposing planarians to IMI (Imidagold 700 WG®) at concentrations ranging from 50 mg a.
View Article and Find Full Text PDFIntegr Comp Biol
July 2025
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States.
In aquatic ecosystems, freshwater planarians (Dugesia spp.) function as predators, employing specialized adaptations for capturing live prey. This exploratory study examines the predatory interactions between the freshwater planarian Dugesia spp.
View Article and Find Full Text PDFGenes (Basel)
June 2025
Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, China.
Background: The genus (Platyhelminthes: Tricladida) includes a large diversity of free-living freshwater flatworms and is important for studies on regeneration and evolution. This study aims to describe a newly discovered asexual planarian species from southern China and explore its genetic characteristics and regenerative abilities.
Methods: An integrative taxonomic analysis was conducted using morphology, karyology, histology, molecular phylogeny (18S, 28S, COI, mitogenome), and genome size estimation via flow cytometry.
Aquat Toxicol
August 2025
Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande 1749-016, Lisboa, Portugal; Centre for Ecology, Evolution and Environmental Changes (cE3c) & CHANGE - Global Change and Sustainability Institute, Faculdade de Ciências, Universidade de Lisboa, Campo Grande
Due to their potential ecological impacts, environmental contamination by microplastics (MPs) has gained significant attention. This study investigated the adverse effects of dietary exposure to three distinct polymer types of MPs with similar size-aliphatic polyurethane (PU), polyethylene-polytetrafluoroethylene composite (PE-PTFE), and high-density polyethylene-polytetrafluoroethylene-alumina nanocomposite (HDPE-PTFE-alumina) on the freshwater planarian Girardia tigrina. Dietary exposure was simulated to assess the effects of MPs on the organisms' defense systems and locomotor behavior.
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