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The use of optoacoustic imaging takes advantage of the photoacoustic effect to generate high-contrast, high-resolution medical images at penetration depths of up to 5 cm. Multispectral optoacoustic tomography (MSOT) is a type of optoacoustic imaging system that has seen promising preclinical success with a recent emergence into the clinic. Multiwavelength illumination of tissue allows for the mapping of multiple chromophores, which are generated endogenously or exogenously. However, translation of MSOT to the clinic is still in its preliminary stages. For successful translation, MSOT requires refinement of probes and data-acquisition systems to tailor to the human body, along with more intuitive, real-time visualization settings. The possibilities of optoacoustic imaging, namely MSOT, in the clinic are reviewed here. ©RSNA, 2020.
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http://dx.doi.org/10.1148/rycan.2020200066 | DOI Listing |
J Mater Chem B
September 2025
State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, College of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Adenosine triphosphate (ATP) is a critical biomolecule in cellular energy metabolism, with abnormal levels in the bloodstream linked to pathological conditions such as ischemia, cancer, and inflammatory disorders. Accurate and real-time detection of ATP is essential for early diagnosis and disease monitoring. However, conventional biochemical assays and other techniques suffer from limitations, including invasive sample collection, time-consuming procedures, and the inability to provide dynamic, monitoring.
View Article and Find Full Text PDFSci Adv
August 2025
Institute of Biological and Medical Imaging, Bioengineering Center, Helmholtz Zentrum München, Neuherberg, Germany.
Hyperspectral optoacoustic microscopy (OAM) enables obtaining images with label-free biomolecular contrast, offering excellent perspectives as a diagnostic tool to assess freshly excised and unprocessed biological samples. However, time-consuming raster scanning image formation currently limits the translation potential of OAM into the clinical setting, for instance, in intraoperative histopathological assessments, where micrographs of excised tissue need to be taken within a few minutes for fast clinical decision-making. Here, we present a non-data-driven computational framework tailored to enable fast OAM by rapid data acquisition and model-based image reconstruction, termed Bayesian raster-computed optoacoustic microscopy (BayROM).
View Article and Find Full Text PDFJ Transl Med
August 2025
Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.
Background: Anti-angiogenic therapy is a clinically validated method for cancer treatment. It was previously revealed that concurrent targeting of angiogenic and death receptor signaling pathways by a multivalent DR5-specific cytokine TRAIL variant DR5-B genetically fused with the effector peptides, SRH-DR5-B-iRGD, enhances solid tumor suppression and prolongs survival. The SRH peptide is aimed at blocking the tumor neoangiogenesis by preventing activation of the VEGFR2 receptor, while the iRGD peptide interferes with the activation of integrin αβ, and enhances the tumor penetration.
View Article and Find Full Text PDFPhotoacoustics
October 2025
Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 300044, Taiwan.
Spherical arrays are commonly used in volumetric optoacoustic tomography for optimal data acquisition. Yet, such a configuration poses challenges for integration with pulse-echo ultrasound used for image guidance, which relies on different signal acquisition and image formation strategies. A potential solution entails the incorporation of linear array segments into the spherical array to enable hybrid 3D optoacoustic and 2D ultrasound (OPUS) modes.
View Article and Find Full Text PDFBiomed Opt Express
August 2025
DTU Electro, Technical Universisty of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Multi-spectral optoacoustic microscopy (MS-OAM) requires high-performance light sources capable of delivering multiple intense spectral lines precisely matched to the absorption characteristics of selected biomolecules. We present a gas-filled anti-resonant hollow-core fiber (ARHCF) laser source optimized for near-infrared (NIR) MS-OAM. The hydrogen (H)-filled ARHCF laser emits multiple spectral lines with high pulse energy and narrow linewidths (<0.
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