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A jumbo bacteriophage, , infecting pv. oryzae was isolated from rice fields in China. Here, we report the complete genome sequence of this phage, revealing that it had a linear double-stranded DNA (dsDNA) molecule 232,104 bp long, with a G+C content of 58%. It has 251 annotated protein-coding sequences.
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http://dx.doi.org/10.1128/MRA.01072-20 | DOI Listing |
J Biomed Sci
August 2025
Clinical Microbiology Laboratory, Medical School, Attikon University Hospital, National and KapodistrianUniversity of Athens, Rimini 1, Haidari, Athens, Greece.
Background: Therapeutic options against metallo-β-lactamase producing P. aeruginosa (MBL-PA) are limited due to multi-drug resistance. A jumbo phage isolated from wastewater in Greece was characterized microbiologically and genetically and evaluated for its potential as a therapeutic agent alone or in combination with antibiotics in an experimental thigh infection mouse model.
View Article and Find Full Text PDFMicrobiol Resour Announc
July 2025
Laboratory of Veterinary Biochemistry, Rakuno Gakuen University School of Veterinary Medicine, Ebetsu, Japan.
Jumbo phages have potentials for uncovering phage diversity. Here, we report the complete genome sequences of two jumbo phages, ΦNK1 and ΦBrmt, isolated from waste water in Japan. To explore their molecular characteristics, whole phage genomes were sequenced and assembled via the short- and long-read platforms.
View Article and Find Full Text PDFFront Cell Infect Microbiol
June 2025
Institute of Microbiology, Braunschweig University of Technology, Braunschweig, Germany.
is an opportunistic pathogen causing severe infections of the lung, burn wounds and eyes. Due to its intrinsic high antibiotic resistance the bacterium is difficult to eradicate. A promising therapeutic option is the use of -specific bacteriophages.
View Article and Find Full Text PDFMicrobiol Resour Announc
June 2025
Floral and Nursery Plants Research Unit, U.S. National Arboretum, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland, USA.
A -infecting jumbo phage was isolated from soil in Maryland, USA. The complete genome sequence was determined to be 225,638 bp and has a G+C content of 51.8%.
View Article and Find Full Text PDFCell Host Microbe
April 2025
School of Biological Sciences, University of California, San Diego, La Jolla, San Diego, CA 92093, USA. Electronic address:
Many eukaryotic viruses require membrane-bound compartments for replication, but no such organelles are known to be formed by prokaryotic viruses. Bacteriophages of the Chimalliviridae family sequester their genomes within a phage-generated organelle, the phage nucleus, which is enclosed by a lattice of the viral protein ChmA. We show that inhibiting phage nucleus formation arrests infections at an early stage in which the injected phage genome is enclosed within a membrane-bound early phage infection (EPI) vesicle.
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