Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Objectives: We validate the use of a lateral flow immunoassay (LFI) intended for rapid screening and qualitative detection of anti-SARS-CoV-2 IgM and IgG in serum, plasma, and whole blood, and compare results with ELISA. We also seek to establish the value of LFI testing on blood obtained from a capillary blood sample.
Methods: Samples collected by venous blood draw and finger stick were obtained from patients with SARS-CoV-2 detected by RT-qPCR and control patients. Samples were tested with Biolidics 2019-nCoV IgG/IgM Detection Kit lateral flow immunoassay, and antibody calls were compared with ELISA.
Results: Biolidics LFI showed clinical sensitivity of 92% with venous blood at 7 days after PCR diagnosis of SARS-CoV-2. Test specificity was 92% for IgM and 100% for IgG. There was no significant difference in detecting IgM and IgG with Biolidics LFI and ELISA at D0 and D7 (p = 1.00), except for detection of IgM at D7 (p = 0.04). Capillary blood of SARS-CoV-2 patients showed 93% sensitivity for antibody detection.
Conclusions: Clinical performance of Biolidics 2019-nCoV IgG/IgM Detection Kit is comparable to ELISA and was consistent across sample types. This provides an opportunity for decentralized rapid testing and may allow point-of-care and longitudinal self-testing for the presence of anti-SARS-CoV-2 antibodies.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647890 | PMC |
| http://dx.doi.org/10.1016/j.jim.2020.112909 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Rapid detection of carbapenemases in multiresistant Gram-negative strains: evaluation of two tests.
Microbiol Spectr
September 2025
Institute for Medical Laboratory Diagnostics, Helios University Hospital, Witten/Herdecke University, Wuppertal, Germany.
Carbapenem-resistant organisms (CRO) have rapidly spread worldwide in recent years, posing a significant challenge to both human health and healthcare systems. Timely and accurate detection of CRO, especially carbapenemase-producing and non-fermenters, is crucial for clinical prevention and treatment of these infections. In the present study, we subjected more than 114 multidrug-resistant Gram-negative and non-fermenters to two tests for the timely detection of carbapenemases.
View Article and Find Full Text PDFAre Sex-Specific Cutoffs Needed With a Next-Generation Urine Tenofovir Lateral Flow Assay for Antiretroviral Adherence Monitoring?
Open Forum Infect Dis
September 2025
Department of Epidemiology, University of Washington, Seattle, Washington, USA.
Accurate point-of-care tools are needed to detect early nonadherence to daily HIV regimens and support timely transitions to long-acting options. Emerging evidence suggests that females may require higher adherence than males to achieve equivalent protection. Our next-generation urine tenofovir assay showed high accuracy across sexes but lower urine drug levels among female participants.
View Article and Find Full Text PDFDevelopment and validation of a recombinant Rap1-based lateral flow immunoassay for rapid serodiagnosis of bovine babesiosis in Kazakhstan.
Vet World
July 2025
Laboratory of Immunochemistry and Immunobiotechnology, National Center for Biotechnology, 010000, Astana, Kazakhstan.
Background And Aim: Bovine babesiosis, caused by , poses significant economic challenges to Kazakhstan's cattle industry. Early and accurate detection is crucial for interrupting transmission cycles, particularly in regions lacking advanced diagnostic infrastructure. This study aimed to develop a rapid lateral flow immunoassay (LFIA) using a recombinant C-terminal fragment of the recombinant rhoptry-associated protein 1 (rRap1) antigen for the serodiagnosis of bovine babesiosis.
View Article and Find Full Text PDFRecent advances in techniques used in the diagnosis of lumpy skin disease: a review.
J Vet Diagn Invest
September 2025
Biology Department; Faculty of Sciences, Umm Al-Qura University, Makkah, Saudi Arabia.
Lumpy skin disease (LSD) is a viral disease that affects livestock and is caused by the lumpy skin disease virus (LSDV). An outbreak of LSD in any country can lead to acute economic damage for livestock owners. The significance of prompt and accurate diagnosis in managing this viral disease cannot be overstated.
View Article and Find Full Text PDFReduced model aided fluid-structure interaction design framework for shunt systems.
Med Eng Phys
October 2025
Department of Engineering Science, University of Oxford, United Kingdom. Electronic address:
Traditionally, clinical devices are designed, tested and improved through lengthy and expensive laboratory experiments and clinical trials [1]. More recently, computational methods have allowed for rapid testing, speeding up the design process and enabling far more complete searches of design space. While computational models cannot fully capture the complexities of biological systems, they provide valuable insights into crucial underlying mechanisms, such as the effects of fluid-structure interactions (FSIs).
View Article and Find Full Text PDF