Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Purpose: To develop a practical technique for visualizing and quantifying retinal ganglion cell (RGC) axon bundles .
Methods: We applied visible-light optical coherence tomography (vis-OCT) to image the RGC axon bundles, referred to as vis-OCT fibergraphy, of healthy wild-type C57BL/6 mice. After vis-OCT imaging, retinas were flat-mounted, immunostained with anti-beta-III tubulin (Tuj1) antibody for RGC axons, and imaged with confocal microscopy. We quantitatively compared the RGC axon bundle networks imaged by vis-OCT and confocal microscopy using semi-log Sholl analysis.
Results: Side-by-side comparison of confocal microscopy and vis-OCT confirmed that vis-OCT fibergraphy captures true RGC axon bundle networks. The semi-log Sholl regression coefficients extracted from vis-OCT fibergrams (3.7 ± 0.8 mm) and confocal microscopy (3.6 ± 0.3 mm) images also showed good agreement with each other ( = 6).
Conclusions: We demonstrated the feasibility of using vis-OCT fibergraphy to visualize RGC axon bundles. Further applying Sholl analysis has the potential to identify biomarkers for non-invasively assessing RGC health.
Translational Relevance: Our novel technique for visualizing and quantifying RGC axon bundles provides a potential measurement tool for diagnosing and tracking the progression of optic neuropathies.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7552935 | PMC |
| http://dx.doi.org/10.1167/tvst.9.11.11 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the role of VAV2 rare variants in primary open-angle glaucoma: genetic insights and pathophysiological mechanisms.
Sci China Life Sci
August 2025
Sichuan Provincial Key Laboratory for Human Disease Gene Study and the Center for Medical Genetics, Department of Laboratory Medicine, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu,
Primary open-angle glaucoma (POAG) is the leading cause of irreversible blindness worldwide, primarily due to the degeneration of retinal ganglion cells (RGCs). In this study, we reported vav guanine nucleotide exchange factor 2 (VAV2) as a POAG-associated gene. Through whole exome sequencing (WES) of 398 Han Chinese POAG patients and 2,010 controls, we discovered nine rare VAV2 variants linked to POAG (P_burden=1.
View Article and Find Full Text PDFIntravitreal NHS-Biotin Injection and Immunohistochemistry to Label and Image Protein Transport in the Mouse Optic Nerve.
Bio Protoc
August 2025
The Scripps Research Institute, Neuroscience Department and Dorris Neuroscience Center, La Jolla, CA, USA.
The process of moving proteins and organelles along the axon is essential for neuronal survival and function, ensuring proper communication between the cell body and distant synapses. The efficient and precise delivery of proteins via axon transport is critical for processes ranging from synaptic plasticity and neurotransmission to neuronal growth and maintenance. However, the identities of all the transported proteins have only recently begun to be investigated.
View Article and Find Full Text PDFOptimized in vivo two-photon imaging reveals the essential role of the contralateral eye in functional optic nerve regeneration in zebrafish larvae.
Eye Vis (Lond)
August 2025
State Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, China.
Background: The visual pathway, consisting of the eye, optic nerve, and brain, serves as a valuable model for studying neural regeneration. The exceptional regenerative capacity of the zebrafish visual system enables detailed investigation of neural repair mechanisms in vivo. Although the transparency of zebrafish larvae permits real-time imaging of axonal regeneration following transection, previous methodological limitations such as pigment interference and suboptimal imaging protocols have hindered high-resolution analyses of structural recovery and cellular interaction throughout the entire visual pathway after optic nerve injury.
View Article and Find Full Text PDFGlaucoma-associated Optineurin mutations increase transcellular degradation of mitochondria in a vertebrate optic nerve.
Elife
August 2025
Department of Ophthalmology and Vision Science, University of California, Davis, Davis, United States.
We previously described a process whereby mitochondria shed by retinal ganglion cell (RGC) axons are transferred to and degraded by surrounding astrocytes in the optic nerve head of mice. Since the mitophagy receptor Optineurin (OPTN) is one of few large-effect glaucoma genes and axonal damage occurs at the optic nerve head in glaucoma, here we explored whether OPTN mutations perturb the transcellular degradation of mitochondria. Live-imaging of optic nerves revealed that diverse human mutant but not wildtype OPTN increase stationary mitochondria and mitophagy machinery and their colocalization within, and in the case of the glaucoma-associated OPTN mutations also outside of, RGC axons.
View Article and Find Full Text PDFTumor Necrosis Factor Receptor Superfamily Member 12A Enhances Retinal Ganglion Cell Survival and Promotes Axon Regeneration.
FASEB J
August 2025
Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, China.
Regeneration of retinal ganglion cells (RGCs) in adult mammals is highly limited following injury. To uncover transcriptional pathways that enhance this process, we analyzed single-cell transcriptomic profiles of RGCs in mice after optic nerve crush (ONC) and identified potential targets for neuroprotection and axonal regeneration. scRNA-seq revealed that Tumor necrosis factor receptor superfamily member 12A (Tnfrsf12a, also known as Fn14) is enriched in biological processes linked to axonal regeneration in αRGCs/ipRGCs.
View Article and Find Full Text PDF