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Article Abstract
RFLP analysis of the flagellin (A) gene was compared using three different restriction endonucleases I, I and II to determine the genetic diversity among 43 isolates of poultry origin from the same geographical area. A gene was amplified in all the isolates and RFLP analysis showed variations. -based RFLP was found most efficient in discriminating isolates by generating 15 different -RFLP patterns with discriminatory index (D.I) of 0.9258 whereas II produced seven -RFLP patterns (D.I .= 0.8427). While I enzyme produced only six f-RFLP patterns (D.I .= 0.6977). The discrimination of -RFLP was comparable to discrimination given by -RFLP analysis, which is generally used to study A gene RFLP.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386692 | PMC |
| http://dx.doi.org/10.1016/j.vas.2018.06.003 | DOI Listing |
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