Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Analytical sensitivity for SARS-CoV-2 detection is a key performance metric for the evaluation of viral detection assays. We determined analytical limits of detection for seven SARS-CoV-2 assays using serial dilutions of pooled patient material quantified with droplet digital PCR. Limits of detection ranged from ≤10 to 74 copies/ml for commercial high-throughput laboratory analyzers (Roche Cobas, Abbott m2000, and Hologic Panther Fusion) and 167 to 511 copies/ml for sample-to-answer (DiaSorin Simplexa, GenMark ePlex) and point-of-care instruments (Abbott ID NOW). The CDC assay yielded limits of detection ranging from 85 to 499 copies/ml, depending on the extraction method and thermocycler used. These results can help to inform the assay choice for testing approaches to manage the current COVID-19 outbreak.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448668 | PMC |
| http://dx.doi.org/10.1128/JCM.01535-20 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nitrous oxide (N2O) recreational use is increasing across Germany - a survey of the German neurological society among practicing neurologists.
Neurol Res Pract
September 2025
German Neurological Society, Berlin, Germany.
Background: Recreational nitrous oxide (NO) abuse has become increasingly prevalent, raising concerns about associated health risks. In Germany, the lack of reliable data on NO consumption patterns limits the development of effective public health interventions. This study aims to address this knowledge gap by examining trends, determinants, and health consequences of NO abuse in Germany.
View Article and Find Full Text PDFPre-employment urine drug screening: examining trends in THC-COOH positivity rates post-legalization of recreational cannabis in California - a retrospective review.
J Occup Med Toxicol
September 2025
Occupational Medicine, Antioch Medical Center, Kaiser Permanente, 4501 Sand Creek Road, Antioch, CA, 94531, USA.
Background: This study examines trends in delta-9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) positivity rates in pre-employment urine drug screenings at a single university-based hospital occupational medicine clinic from 2017 to 2022, following California's recreational cannabis legalization in 2016, with sales beginning officially on January 1, 2018.
Methods: Retrospective analysis of 21,546 de-identified urine drug screenings from 2017 to 2022 was conducted. Initial screening used instant urine drug immunoassays (50 ng/mL cutoff for THC-COOH), followed by confirmatory gas chromatography-mass spectrometry (15 ng/mL cutoff).
Construction of an Ag-functionalized structural color hydrogel sensor for colorimetric detection of glutathione.
Mikrochim Acta
September 2025
Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
An Ag-functionalized structural color hydrogel (Ag-SCH) sensor is constructed for colorimetric detection of glutathione (GSH). The hydrogel is prepared by using the coordination of Ag and 1-vinylimidazole (1-VI) as cross-linking network. GSH acts as a competitive ligand to break the coordination between Ag and 1-VI, leading to the expansion and structural color change of the hydrogel.
View Article and Find Full Text PDFContamination profile and potential human health risks of radon in groundwater of southwest region of Punjab, India.
Environ Geochem Health
September 2025
Environmental Hydrology Division, National Institute of Hydrology, Roorkee, 247667, India.
Radon (Rn) is a naturally occurring radioactive gas produced by the decay of uranium-bearing minerals in rocks and soils. Long-term exposure to elevated radon levels in drinking water is associated with an increased risk of stomach and lung cancers. This study aims to assess the concentration of radon in groundwater and evaluate its potential health risks in six cancer-affected districts, i.
View Article and Find Full Text PDFEmerging strategies, applications and challenges of targeting NAD in the clinic.
Nat Aging
September 2025
Department of Clinical Molecular Biology, University of Oslo and Akershus University Hospital, Lørenskog, Norway.
Beyond their classical functions as redox cofactors, recent fundamental and clinical research has expanded our understanding of the diverse roles of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) in signaling pathways, epigenetic regulation and energy homeostasis. Moreover, NAD and NADP influence numerous diseases as well as the processes of aging, and are emerging as targets for clinical intervention. Here, we summarize safety, bioavailability and efficacy data from NAD-related clinical trials, focusing on aging and neurodegenerative diseases.
View Article and Find Full Text PDF