Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
A Gram-stain-negative, long-rod-shaped and facultative aerobic bacterium, designated HB-1, was isolated from a round hay bale at the Kansas State University Beef Stocker Unit. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that strain HB-1 clustered within the genus and its closest relatives were A1-9 (98.0 %), YJ-T1-11 (98.0 %), JS43 (97.8 %), DSM 3857 (97.5 %) and Orc-4 (96.9 %). Additional phylogenomic analysis also indicated that strain HB-1 belongs to the genus . The draft genome of strain HB-1 had a total length of 4.23 Mbp and contained 4071 protein-coding genes. The average nucleotide identity values between the genomes of strain HB-1 and the three most-related type strains ranged from 77.5 to 78.1 %. The DNA G+C content of strain HB-1 was 63.7 mol%. The novel strain grew at 10-37 °C, pH 5-10 and with 0-2 % NaCl. Oxidase and catalase activities were positive. Cells were 0.3-0.4 µm wide, 3.0-7.0 µm long and usually found in pairs or chains of cells. The major respiratory quinone of strain HB-1 was Q-10 (90 %), with a minor amount of Q-9 (10 %). The major fatty acids were C ω7 (54.6 %) and C (18.2 %). On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain HB-1 (=DSM 109828=ATCC TSD-211) is considered to represent a novel species of the genus , for which the name sp. nov. is proposed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/ijsem.0.004276 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Engineering with Effective Heme Supply for the Synthesis of High-Activity Hemoglobins and Myoglobins.
J Agric Food Chem
June 2025
Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
Hemoglobin (Hb) and myoglobin (Mb) have been extensively employed as oxygen carriers, iron supplements, and food additives, but the microbial synthesis of functional Hb and Mb remains a challenge. Herein, we engineered a strain with efficient heme supply for producing active Hb and Mb. Through the iterative multimodule strategy, including improvement of precursor supply (5-aminolevulinate), redirection of unstable hydroxymethylbilane, selection of the coproporphyrin-dependent pathway as the superior downstream route, enhancement of rate-limiting steps, and prevention of heme secretion, the heme titer was increased by 90.
View Article and Find Full Text PDFAntimicrobial susceptibility testing of Clostridioides difficile: a dual-site study of three different media and three therapeutic antimicrobials.
Clin Microbiol Infect
June 2025
European Society of Clinical Microbiology and Infectious Diseases Study Group on Clostridioides difficile, Basel, Switzerland; Experimental Bacteriology Research Group, Leiden University Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: w.
Objectives: Increasing resistance to antimicrobials used for the treatment of Clostridioides difficile infections necessitates reproducible antimicrobial susceptibility testing. Current guidelines take a one-size-fits-all approach and/or offer limited guidance. We investigated how the choice of medium affects measured MIC values across two sites.
View Article and Find Full Text PDFsp. nov., a denitrifying bacterium, isolated from pond water for .
Int J Syst Evol Microbiol
June 2024
Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Key Laboratory of Agricultural Microbiome (MARA), Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied
A novel Gram-stain-negative strain, designated JM10B15, was isolated from pond water for collected from Jiangmen City, Guangdong province, south PR China. Cells of the strain were aerobic, rod-shaped, and motile by lateral flagella. JM10B15 could grow at 15-40 °C, pH 6.
View Article and Find Full Text PDFsp. nov., isolated from conserved forages.
Int J Syst Evol Microbiol
July 2020
Department of Civil Engineering, Kansas State University, Manhattan, KS 66503, USA.
A Gram-stain-negative, long-rod-shaped and facultative aerobic bacterium, designated HB-1, was isolated from a round hay bale at the Kansas State University Beef Stocker Unit. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that strain HB-1 clustered within the genus and its closest relatives were A1-9 (98.0 %), YJ-T1-11 (98.
View Article and Find Full Text PDFCRISPR-Cpf1-Assisted Engineering of Corynebacterium glutamicum SNK118 for Enhanced L-Ornithine Production by NADP-Dependent Glyceraldehyde-3-Phosphate Dehydrogenase and NADH-Dependent Glutamate Dehydrogenase.
Appl Biochem Biotechnol
July 2020
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Here, Corynebacterium glutamicum SNK118 was metabolically engineered for L-ornithine production through CRISPR-Cpf1-based genome manipulation and plasmid-based heterologous overexpression. Genes argF, argR, and ncgl2228 were deleted to block the degradation of L-ornithine, eliminate the global transcriptional repression, and alleviate the competitive branch pathway, respectively. Overexpression of CsgapC (NADP-dependent glyceraldehyde 3-phosphate dehydrogenases gene from Clostridium saccharobutylicum DSM 13864) and BsrocG (NADH-dependent glutamate dehydrogenase gene from Bacillus subtilis HB-1) resulted markedly increased ornithine biosynthesis.
View Article and Find Full Text PDF