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Background: The aim of this work was to evaluate the microscopic characteristics through polarized light microscopy, scanning electron microscopy and the mineral content of circumpulpal dentin of irradiated (IT) and non-irradiated teeth (NIT), with deep caries that reached the root canal.
Material And Methods: A total of 25 IT were analyzed macroscopically, and radiographed. 5 NIT were used as controls. Two 100-μm-thick sections, per specimen, were evaluated in a polarized light microscopy and a scanning electron microscope. Demographics and clinical data were collected.
Results: The results did not demonstrate distinct morphology of the IT compared with NIT. Mineral content values by weight percentage of Ca and P were also similar, corresponding to 66.65% and 33.21% in the IT and 66.60% and 33.29% in the NIT. The Ca/P ratio did not show statistical differences between groups being respectively 2.74 and 2.72, in the IT and NIT (> 0.05).
Conclusions: Radiotherapy does not change morphology and mineral content of circumpulpal dentin in IT. Root canal, radiation-related caries, polarization microscopy, scanning electron microscopy, radiotherapy, oral neoplasms, dentin.
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http://dx.doi.org/10.4317/jced.56584 | DOI Listing |
Sci Rep
July 2025
Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 1011 North University Ave, Ann Arbor, MI, 48109, USA.
Dentin sialophosprotein (DSPP) is cleaved into the N-terminal dentin sialoprotein (DSP) proteoglycan and the C-terminal dentin phosphoprotein (DPP; the most acidic proteins in humans). To define the functions of DSP and DPP, we generated a Dspp mouse model using CRISPR/Cas9 technology and compared tooth mineralization in Dspp and Dspp mice in the C57BL/6 background. In both mice, the initiation of dentin mineralization was associated with matrix vesicles.
View Article and Find Full Text PDFInt J Paleopathol
December 2023
Massachusetts General Hospital and Harvard Medical School, 55 Fruit St, Boston, MA 02114, United States.
Objective: Cortisol is a glucocorticoid hormone produced by the hypothalamic-pituitary-adrenal axis that is regularly assessed in modern human and non-human populations in saliva, blood, and hair as a measure of stress exposure and stress reactivity. While recent research has detected cortisol concentrations in modern and archaeological permanent dental tissues, the present study assessed human primary (deciduous) teeth for cortisol concentrations.
Materials And Methods: Fifty-one dentine and enamel samples from nine modern and 10 archaeological deciduous teeth were analyzed for cortisol concentrations via enzyme-linked immunosorbent assay (ELISA).
Radiat Environ Biophys
May 2023
Department of Dentomaxillofacial Surgery, Faculty of Dentistry, Çanakkale Onsekiz Mart University, Çanakkale, 17100, Turkey.
The aims of the study were to analyze the effects of therapeutic radiation on human root dentin samples from the aspect of possible alterations in crystallinity, micro-morphology, and composition. Fifty-six root dentin specimens were divided into seven groups (0, 10, 20, 30, 40, 50, and 60 Gy). Scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD) analyses were performed on pulpal surfaces of root dentin after being irradiated by 6MV photon energy.
View Article and Find Full Text PDFBone
February 2021
Division of Biosciences, College of Dentistry, The Ohio State University, Columbus, OH, USA. Electronic address:
ALPL encodes tissue-nonspecific alkaline phosphatase (TNAP), an enzyme expressed in bone, teeth, liver, and kidney. ALPL loss-of-function mutations cause hypophosphatasia (HPP), an inborn error-of-metabolism that produces skeletal and dental mineralization defects. Case reports describe widely varying dental phenotypes, making it unclear how HPP comparatively affects the three unique dental mineralized tissues: enamel, dentin, and cementum.
View Article and Find Full Text PDFStomatologiia (Mosk)
July 2020
Russian Ilizarov Scientific Center «Restorative Traumatology and Orthopaedics» of Ministry of Healthcare of the Russian Federation, Kurgan, Russia.
The study was aimed to conduct a morphological assessment of the influence exerted by a certain method of activation of endodontic rinsing liquid on the root dentin structure and canal cleaning effectiveness in an ex vivo experiment. The study was performed on 20 single-rooted teeth removed due to complicated caries in people aged 25-44 years. A devised method of vacuum-jet irrigation was used to carry out the final antiseptic treatment and to activate a rinsing liquid in the group No.
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