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http://dx.doi.org/10.1074/jbc.AAC120.013773 | DOI Listing |
NAR Genom Bioinform
September 2025
DNA Repair and Recombination Laboratory, St Vincent's Institute of Medical Research, Fitzroy VIC 3065, Australia.
Meiotic crossovers promote correct chromosome segregation and the shuffling of genetic diversity. However, the measurement of crossovers remains challenging, impeding our ability to decipher the molecular mechanisms that are necessary for their formation and regulation. Here we demonstrate a novel repurposing of the single-nucleus Assay for Transposase Accessible Chromatin with sequencing (snATAC-seq) as a simple and high-throughput method to identify and characterize meiotic crossovers from haploid testis nuclei.
View Article and Find Full Text PDFNat Chem Biol
September 2025
Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Nat Struct Mol Biol
August 2025
Laboratory of Chromatin Biology, Max-Planck Institute of Biochemistry, Martinsried, Germany.
J Chem Theory Comput
September 2025
Université Paris Cité, CNRS, Laboratoire de Biochimie Théorique, 13 rue Pierre et Marie Curie, 75005 Paris, France.
While divalent ions are known to be involved in key biological processes such as RNA folding or DNA-histone interactions, these interactions are poorly captured in molecular dynamics simulations with empirical force fields, which suffer from strong overbinding artifacts. Hence, there is a strong need for improved descriptions of (divalent) ions in nucleic acid simulations. In this work, we explore the possibility to improve ion-binding properties of the popular Amber-OL15 force field using the Electronic Continuum Correction (ECC) approach, which includes electronic polarization through charge scaling, limited here to the phosphate backbone.
View Article and Find Full Text PDFNat Chem Biol
August 2025
Max Planck Institute for Biology of Ageing, Cologne, Germany.