Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The ocular surface is subjected to regular wear and tear due to various environmental factors. Exposure to UV-C radiation constitutes an occupational health hazard. Here, we demonstrate the exposure of primary stem cells from the mouse ocular surface to UV-C radiation. Reactive oxygen species (ROS) formation is the readout of the extent of oxidative stress/damage. In an experimental in vitro setting, it is also essential to assess the percentage of dead cells generated due to oxidative stress. In this article, we will demonstrate the 2',7'-Dichlorofluoresceindiacetate (DCFDA) staining of UV-C exposed mouse primary ocular surface stem cells and their quantification based on the fluorescent images of DCFDA staining. DCFDA staining directly corresponds to ROS generation. We also demonstrate the quantification of dead and live cells by simultaneous staining with propidium iodide (PI) and Hoechst 3332 respectively and the percentage of DCFDA (ROS positive) and PI positive cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3791/59924 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Association of Dry Eye and Corneal Ulcers With Collagen Vascular Diseases in a Medicare Population.
Cornea
September 2025
Icahn School of Medicine at Mount Sinai, Department of Ophthalmology, New York, NY.
Purpose: There is a lack of research on the extent to which non-Sjögren collagen vascular diseases affect the ocular surface. This study aims to understand the associations between collagen vascular diseases and dry eye and corneal ulcers.
Methods: This study analyzed a random 5% sample of national Medicare beneficiaries from 2011 to 2015 and included claims for those with collagen vascular diseases and either dry eye or corneal ulcers (n = 2,688,114).
Altered Corneal T-Cell Motility and Sensory Nerve Features in Older Adults With Human Immunodeficiency Virus Infection.
Invest Ophthalmol Vis Sci
September 2025
Department of Optometry and Vision Sciences, The University of Melbourne, Melbourne, Australia.
Purpose: To characterize corneal immune cell morphodynamics and nerve features, and define the in vivo immune landscape in older adults with human immunodeficiency virus (HIV) receiving antiretroviral therapy (ART), relative to healthy age-matched adults.
Methods: In this cross-sectional study, 16 HIV-positive individuals receiving ART and 15 age-matched controls underwent ocular surface examinations and functional in vivo confocal microscopy (Fun-IVCM). Time-lapsed videos were created to analyze corneal immune cells (T cells, dendritic cells [DCs], macrophages).
Orbital Presentation of Eosinophilic Granulomatosis with Polyangiitis: An Interventional Case Report and Literature Review.
J Ophthalmic Vis Res
August 2025
Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Purpose: To report a case of eosinophilic granulomatosis with polyangiitis (EGPA) initially presenting as orbital involvement, describe its successful management, and provide a comprehensive literature review.
Case Report: A 33-year-old female patient presented with swelling, redness, tenderness, and a mass under the left upper eyelid for one month. Upper lid eversion showed a multilobulated lesion in the subconjunctival area of the same region.
Depression, anxiety, stress, and dry eye disease among Gazan undergraduate health sciences and nursing students: a structural equation modeling study.
Cont Lens Anterior Eye
September 2025
Keele University, Stafforshire, UK.
Purpose: To investigate associations between dry eye disease (DED) symptoms and psychological distress (depression, anxiety, stress) among undergraduate health sciences and nursing students in the Gaza Strip during the 2023-2025 conflict period.
Methods: A cross-sectional study used convenience sampling via WhatsApp and face-to-face interviews between 4 February and 29 April 2025. Participants completed a demographic form, the Arabic Ocular Surface Disease Index (OSDI), and the Arabic Depression Anxiety Stress Scale-8 (DASS-8).
Orbital Exenteration Trends at a Single Institution From 2011 to 2024.
Ophthalmic Plast Reconstr Surg
September 2025
Department of Ophthalmology, Bascom Palmer Eye Institute.
Purpose: The primary objective was to investigate the trends in orbital exenteration rates at a large tertiary care center, particularly in the context of recent advancements in immunotherapy, targeted agents, and globe-sparing surgical techniques, which have significantly impacted patient management.
Methods: We conducted a retrospective cohort study at the University of Miami. Patients who underwent orbital exenterations from 2011 to 2024 were identified by obtaining surgical coding data via institutional data brokers and validated through a rigorous surgical chart review.