Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The packaging of molecular constituents inside extracellular vesicles (EVs) allows them to participate in intercellular communication and the transfer of biological molecules, however the role of EVs during bacterial infection is poorly understood. The goal of this study was to examine the effects of ( infection on the biogenesis and composition of EVs derived from the mouse microglia cell line, BV-2. BV-2 cells were cultured in exosome-free media and infected with 0, 1.3 × 10, or 2.6 × 10 colony forming units per milliliter for 72 h. The results indicated that compared with the control group, BV-2 cell viability significantly decreased after infection and BV-2-derived EVs concentration decreased significantly in the infected group. infection significantly decreased chemokine ligand 4 messenger RNA in BV-2-derived infected EVs, compared with the control group ( ≤ 0.05). This study also revealed that heat shock protein 70 ( ≤ 0.05) and heat shock protein 90β ( ≤ 0.001) levels of expression within EVs increased after infection. EV treatment with EVs derived from infection reduced cell viability of BV-2 cells. infection alters the expression of specific proteins and mRNA in EVs. Our study suggests that infection modulates EV biogenesis and composition, which may influence bacterial pathogenesis and infection.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963293 | PMC |
| http://dx.doi.org/10.3390/pathogens8040297 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structure, function and assembly of nuclear pore complexes.
Nat Rev Mol Cell Biol
September 2025
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, USA.
The defining property of eukaryotic cells is the storage of heritable genetic material in a nuclear compartment. For eukaryotic cells to carry out the myriad biochemical processes necessary for their function, macromolecules must be efficiently exchanged between the nucleus and cytoplasm. The nuclear pore complex (NPC) - which is a massive assembly of ~35 different proteins present in multiple copies totalling ~1,000 protein subunits and architecturally conserved across eukaryotes - establishes a size-selective channel for regulated bidirectional transport of folded macromolecules and macromolecular assemblies across the nuclear envelope.
View Article and Find Full Text PDFPurifying recombinant proteins from Nicotiana benthamiana for structural studies.
Nat Protoc
September 2025
Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Structural biology is fundamental to understanding the molecular basis of biological processes. While machine learning-based protein structure prediction has advanced considerably, experimentally determined structures remain indispensable for guiding structure-function analyses and for improving predictive modeling. However, experimental studies of protein complexes continue to pose challenges, particularly due to the necessity of high protein concentrations and purity for downstream analyses such as cryogenic electron microscopy.
View Article and Find Full Text PDFThe lipocone superfamily, a unifying theme in metabolism of lipids, peptidoglycan and exopolysaccharides, inter-organismal conflicts and immunity.
Elife
September 2025
Division of Intramural Research, National Library of Medicine, National Institutes of Health, Bethesda, United States.
Wnt proteins are critical signaling molecules in developmental processes across animals. Despite intense study, their evolutionary roots have remained enigmatic. Using sensitive sequence analysis and structure modeling, we establish that the Wnts are part of a vast assemblage of domains, the Lipocone superfamily, defined here for the first time.
View Article and Find Full Text PDFApplication of droplet digital PCR for the detection of fish DNA in food products.
Food Res Int
November 2025
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy. Electronic address:
Fish is one of the most common causes of food allergy. The global prevalence of fish allergy has increased over the years as a result of the increased fish consumption. In allergic individuals even small amounts of allergen can trigger a life-threatening allergic reaction.
View Article and Find Full Text PDFEffects of Brown Rice, Meal Replacements, and Anti-Obesity Drugs on Mitochondria in Obese Rats.
Diabetes Metab Syndr Obes
September 2025
School of Medical, Indigenous and Health, University of Wollongong, Wollongong, New South Wales, Australia.
Introduction: Obesity remains a critical global health challenge, intricately linked to poor dietary quality, gut microbiota dysbiosis, and mitochondrial dysfunction.
Purpose: This study aimed to investigate the comparative effects of brown rice, meal replacements, and thiazolidinediones on mitochondrial abundance and gut microbiota composition in a rat model of diet-induced obesity.
Methods And Materials: A total of twenty male Sprague Dawley rats were randomly assigned to five groups: control, high-fat high-fructose diet, and three intervention groups receiving the same obesogenic diet supplemented with brown rice, meal replacement, or thiazolidinediones for twelve weeks.