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Six pesticides, azoxystrobin, boscalid, chlorfenapyr, imazalil, isoxathion, and nitenpyram, were simultaneously detected by using a surface plasmon resonance (SPR) immunosensor. The working ranges were 3.5 - 19 ng/mL for azoxystrobin, 4.5 - 50 ng/mL for boscalid, 2.5 - 25 ng/mL for chlorfenapyr, 5.5 - 50 ng/mL for imazalil, 3.5 - 50 ng/mL for isoxathion, and 8.5 - 110 ng/mL for nitenpyram. They showed adequate recovery results in tomato samples: 104 - 116% for azoxystrobin, 94 - 101% for boscalid, 90 - 112% for chlorfenapyr, 96 - 106% for imazalil, 107 - 119% for isoxathion, and 104 - 109% for nitenpyram. The correlation coefficient with liquid chromatography (HPLC or LC-MS/MS) using vegetable samples also agreed well: 0.91 - 0.99 as R without strong bias, except for nitenpyram for which the SPR immunosensor sensitivity was too low. The SPR immunosensor will have high applicability for pesticide residue analyses in vegetable samples.
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http://dx.doi.org/10.2116/analsci.19P333 | DOI Listing |
ACS Infect Dis
August 2025
Laboratório de Espectrometria, Sensores e Biossensores - Department of Chemistry, Federal University of Paraná (UFPR), Curitiba, PR 81530-900, Brazil.
Cryptococcosis is a severe fungal infection, particularly in immunosuppressed individuals, causing over 112,000 HIV-related deaths annually. Early and accurate diagnosis is critical, but current methods often lack the necessary sensitivity, specificity, and accessibility for point-of-care use. A major challenge is identifying highly specific bioreceptors for detecting -specific antibodies.
View Article and Find Full Text PDFMikrochim Acta
July 2025
Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, 518055, China.
The synergy between electroosmotic flow (EOF) and surface plasmon resonance (SPR) offers a transformative approach to overcome diffusion-limited biosensing. However, existing voltage-driven EOF systems face critical trade-offs between high-voltage requirements and biocompatibility. Here, we present a self-powered electroosmosis-enhanced SPR immunosensor that integrates a triboelectric nanogenerator (TENG) with SPR imaging (SPRi) to achieve ultra-sensitive detection of HER2-positive cancer cells.
View Article and Find Full Text PDFBiosens Bioelectron
November 2025
College of Public Health, Zhengzhou University, Henan, Zhengzhou, 450001, China. Electronic address:
Soluble cluster of differentiation 146 (sCD146) is expressed in various immune cells and plays crucial roles in cellular processes linked to tumor cell metastasis, as well as in cell signalling, migration, proliferation, and inflammation. Detecting sCD146 in plasma has become a promising approach for early cancer screening. A rapid and highly specific dual-mode immunosensor that integrates colorimetry and surface plasmon resonance (SPR) techniques was successfully developed in this study.
View Article and Find Full Text PDFTalanta
August 2025
Sorbonne Université, CNRS, Laboratoire de Réactivité de Surface (LRS), F-75005, Paris, France. Electronic address:
We report the design of a smartphone-assisted plasmonic immunosensor and its application to the detection of several food contaminants, namely staphylococcal enterotoxin A (SEA), bovine β-lactoglobulin (BLG), and hen egg white lysozyme (LYSO). Comprehensive characterization of antibodies was performed by ELISA and surface plasmon resonance imaging (SPR-i), demonstrating high affinity and specificity for each target, which supports their potential application in complex food matrices. The immunosensing platform utilizes gold nanoparticle-conjugated antibodies and inexpensive glass slides as single-use chips.
View Article and Find Full Text PDFBiosensors (Basel)
October 2024
Department of Biotechnology, School of Chemical and Life Science, Jamia Hamdard University, New Delhi 110062, India.