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Article Abstract
Neuronal microexons represent the most highly conserved class of alternative splicing events and their timed expression shapes neuronal biology, including neuronal commitment and differentiation. The six-nt microexon 34' is included in the neuronal form of mRNA, which encodes the largest subunit of the basal transcription factor TFIID. In this study, we investigate the tissue distribution of mRNA and protein and the mechanism responsible for its neuronal-specific splicing. Using isoform-specific RNA probes and antibodies, we observe that canonical TAF1 and TAF1-34' have different distributions in the brain, which distinguish proliferating from post-mitotic neurons. Knockdown and ectopic expression experiments demonstrate that the neuronal-specific splicing factor SRRM4/nSR100 promotes the inclusion of microexon 34' into mRNA, through the recognition of UGC sequences in the poly-pyrimidine tract upstream of the regulated microexon. These results show that SRRM4 regulates temporal and spatial expression of alternative mRNAs to generate a neuronal-specific TFIID complex.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948980 | PMC |
| http://dx.doi.org/10.1080/15476286.2019.1667214 | DOI Listing |
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Article Synopsis
- Neuronal microexons, particularly the six-nucleotide microexon 34', play a crucial role in neuronal development by influencing the expression of the transcription factor TFIID.
- Researchers explored how this microexon is specifically spliced in neurons, revealing that two TAF1 isoforms have distinct distributions in the brain, differentiating between actively dividing and mature neurons.
- The splicing factor SRRM4/nSR100 enhances the inclusion of microexon 34' in mRNA, highlighting its importance in producing neuron-specific TFIID complexes during neuronal differentiation.