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The authors apologized for the unfortunate error in figure during publication of the article and they also explained that some of the solid grey graphs in Fig. 5 are intentionally based on the same data. For 8 different surface makers (CD14, CD73, CD34, CD105, CD19, CD90, CD45, HA-DR) in accordance to the guidelines of the manufacturer a panel of 4 different isotype controls were used, corresponding to 4 different fluorescence channels.
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http://dx.doi.org/10.1186/s12951-019-0527-6 | DOI Listing |
J Appl Clin Med Phys
September 2025
Clinical Imaging Physics Group, Duke University Health System, Durham, North Carolina, USA.
Introduction: Medical physicists play a critical role in ensuring image quality and patient safety, but their routine evaluations are limited in scope and frequency compared to the breadth of clinical imaging practices. An electronic radiologist feedback system can augment medical physics oversight for quality improvement. This work presents a novel quality feedback system integrated into the Epic electronic medical record (EMR) at a university hospital system, designed to facilitate feedback from radiologists to medical physicists and technologist leaders.
View Article and Find Full Text PDFJ Nucl Med Technol
September 2025
Institute of Nuclear Medicine, First Faculty of Medicine, Charles University and the General University Hospital in Prague, Prague, Czech Republic;
The aim of the study was to validate a new method for semiautomatic subtraction of [Tc]Tc-sestamibi and [Tc]NaTcO SPECT 3-dimensional datasets using principal component analysis (PCA) against the results of parathyroid surgery and to compare its performance with an interactive method for visual comparison of images. We also sought to identify factors that affect the accuracy of lesion detection using the two methods. Scintigraphic data from [Tc]Tc-sestamibi and [Tc]NaTcO SPECT were analyzed using semiautomatic subtraction of the 2 registered datasets based on PCA applied to the region of interest including the thyroid and an interactive method for visual comparison of the 2 image datasets.
View Article and Find Full Text PDFJ Nucl Med Technol
September 2025
Medical Physics Unit, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
The glomerular filtration rate (GFR) is a key measure of renal function, typically estimated using creatinine-based equations. More precise clearance measurements are obtained with radiotracers, such as [Tc]Tc-diethylenetriaminepentaacetic acid (DTPA), and blood samples over several hours. However, standard plasma clearance methods require labor-intensive plasma preparation, limiting efficiency in clinical practice.
View Article and Find Full Text PDFCell Rep Methods
August 2025
Department of Biomedical Engineering and Computational Biology Program, OHSU, Portland, OR, USA; Knight Cancer Institute, OHSU, Portland, OR, USA. Electronic address:
We present UniFORM, a non-parametric, Python-based pipeline for normalizing multiplex tissue imaging (MTI) data at both the feature and pixel levels. UniFORM employs an automated rigid landmark registration method tailored to the distributional characteristics of MTI, with UniFORM operating without prior distributional assumptions and handling both unimodal and bimodal patterns. By aligning the biologically invariant negative populations, UniFORM removes technical variation while preserving tissue-specific expression patterns in positive populations.
View Article and Find Full Text PDFJ Am Soc Mass Spectrom
September 2025
Chemistry Department, Indiana University, 800 E Kirkwood Ave, Bloomington, Indiana 47405.
In charge detection mass spectrometry (CD-MS) ions are trapped in an electrostatic linear ion trap (ELIT) where they oscillate back and forth through a conducting cylinder. The oscillating ions induce a periodic charge separation that is detected by a charge sensitive amplifier (CSA) connected to the cylinder. The resulting time domain signal is analyzed using short-time Fourier transforms to give the mass-to-charge ratio and charge for each ion, which are then multiplied to give the mass.
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