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Article Abstract

In this study, the effects of strigolactone (SL) on the biomass, lipid content, biochemical properties, and gene transcription of Monoraphidium sp. QLY-1 were examined. The lipid content and lipid productivity increased by 61% and 55% in QLY-1 under 1 μM SL induction compared to the control group, respectively. SL also upregulated the levels of endogenous NO and Ca and lipid biosynthesis gene transcription. Subsequently, the relationship between Ca and nitric oxide (NO) in the regulation of cell growth and lipid accumulation of QLY-1 under SL induction conditions was analysed. An increase in endogenous Ca regulated cell growth and lipid biosynthesis by modulating the levels of NO and lipid biosynthesis-related gene expression. Collectively, this study provided a valuable approach for biofuel production from microalgae under SL induction and demonstrated that there is crucial crosstalk between the Ca and NO signalling in the manipulation of lipid biosynthesis in microalgae under SL treatment.

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http://dx.doi.org/10.1016/j.biortech.2019.121607DOI Listing

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