Enhancing Chlorobenzene Biodegradation by Using a Water-Silicone Oil Biphasic System.

In this study, a water-silicone oil biphasic system was developed to enhance the biodegradation of monochlorobenzene (CB) by LW26. Compared to the single phase, the biphasic system with a suitable silicone oil fraction (/) of 20% allowed a 2.5-fold increase in the maximum tolerated CB concentration. The CB inhibition on LW26 was reduced in the presence of silicone oil, and the electron transport system activity was maintained at high levels even under high CB stress. Adhesion of cells to the water-oil interface at the water side was observed using confocal laser scanning microscopy. Nearly 75% of cells accumulated on the interface, implying that another interfacial substrate uptake pathway prevailed besides that initiated by cells in the aqueous phase. The 8-fold increase in cell surface hydrophobicity upon the addition of 20% () silicone oil showed that silicone oil modified the surface characteristics of LW26. The protein/polysaccharide ratio of extracellular polymeric substances (EPS) from LW26 presented a 3-fold enhancement. These results suggested that silicone oil induced the increase in the protein content of EPS and rendered cells hydrophobic. The resulting hydrophobic cells could adhere on the water-oil interface, improving the mass transfer by direct CB uptake from silicone oil.