A new rapid procedure for simultaneous determination of glyphosate and AMPA in water at sub μg/L level.

Glyphosate is the most used pesticide worldwide and its impact on the environment is becoming increasingly significant. Glyphosate and its main metabolite AMPA are frequently detected in streams and rivers. In this study, an analytical method is presented that combines Ultra-High Performance Liquid Chromatography with mass spectrometry (UHPLC-ESI-MS/MS) for glyphosate and AMPA analysis in environmental water samples. The method was developed starting from an application of Waters Corporation, and involves the use of an alternative derivatizing reagent, the commercially available AccQ·Tag Ultra Derivatization Kit (Waters Corporation, Milford, MA, USA). The kit contains the derivatizing reagent 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). Derivatization takes place directly in the injection vial and no sample pre-concentration is needed. The derivatization is simple, quick and robust, which fits well within the needs of a routine method for the analysis of glyphosate and AMPA. Derivatized glyphosate and AMPA were recorded in positive ion mode. The method demonstrates a good linear relationship in the concentration range from 0.2 μg/L for glyphosate and 0.05 μg/L for AMPA to 100 μg/L and an accurate recovery. The method developed has been successfully applied to the determination of glyphosate and AMPA in 23 runoff water samples collected from a field in the Po Valley (North-East Italy), an agricultural area where glyphosate is widely used.