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Article Abstract
Objectives: Phosphoryl oligosaccharides of calcium (POs-Ca) are a highly soluble calcium source and can keep the solubility of calcium and fluoride ions. The aim of this study was to investigate the effect of calcium (from POs-Ca) and fluoride ions penetrate into subsurface enamel lesions in vitro.
Design: Demineralized bovine enamel slabs were remineralizedin vitro for 24 h at 37 °C with artificial saliva (AS) containing POs-Ca and various fluoride concentrations (0-100 ppm), or AS containing different levels of POs-Ca adjusted to a Ca/P ratio of 0.4-3.0 and fluoride, then were analyzed using Transversal microradiography. From those results, remineralization effects with optimal conditions were compared between POs-Ca and calcium chloride (CaCl). To determine the form of incorporated fluoride, we analyzed the chemical state and local structure of fluorine atoms integrated into enamel subsurface lesions using micro X-ray absorption near-edge structure (μ-XANES) spectroscopy.
Results: A significant mineral recovery rate was observed with POs-Ca and fluoride at 0.5 or 1.0 ppm (n = 6, p < 0.05), as well as a Ca/P molar ratio of 1.67 (n = 5, p < 0.05). Under those conditions, the mineral recovery rate of AS containing POs-Ca (37.9 ± 7.3%) was significantly greater than that of CaCl (15.0 ± 9.6%) (n = 5, mean ± SD, p < 0.05). μ-XANES spectra analysis of the samples indicated that the dominant form of fluorine atoms in enamel subsurface lesions was fluorapatite.
Conclusions: POs-Ca with fluoride-derived diffusion into subsurface enamel lesions facilitated formation of fluorapatite phases.
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| http://dx.doi.org/10.1016/j.archoralbio.2019.03.018 | DOI Listing |
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