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Article Abstract
Collagen is the building component of temporomandibular joint (TMJ) discs and is often affected by inflammation in temporomandibular disorders. The macromechanical properties of collagen are deteriorated by chronic inflammation. However, the mechanism by which inflammation influences disc function remains unknown. The relationship between the ultrastructure and nanomechanical properties of collagen in inflamed discs should be clarified. Seven-week-old female Sprague-Dawley rats were randomly divided into two groups. Chronic TMJ inflammation was induced by intra-articular injection of complete Freund's adjuvant, and samples were harvested after 5 weeks. Picrosirius staining revealed multiple colours under polarized light, which represented alternative collagen bundles in inflamed discs. Using atomic force microscopy scanning, the magnitude of Young's modulus was reduced significantly accompanied with disordered collagen fibril arrangement with porous architecture of inflamed discs. Transmission electron microscopy scanning revealed a non-uniform distribution of collagen fibres, and oversized collagen fibrils were observed in inflamed discs. Fourier transform infrared microspectroscopy revealed a decrease in 1 338 cm/amide II area ratio of collagen in different regions. The peak positions of amide I and amide II bands were altered in inflamed discs, indicating collagen unfolding. Our results suggest that sustained inflammation deteriorates collagen structures, resulting in the deterioration of the ultrastructure and nanomechanical properties of rat TMJ discs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381164 | PMC |
| http://dx.doi.org/10.1038/s41368-018-0036-8 | DOI Listing |
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Article Synopsis
- Collagen is crucial for the structure of TMJ discs, but chronic inflammation from disorders can weaken its mechanical properties, although the exact effects on disc function are not fully understood.
- In a study with Sprague-Dawley rats, chronic TMJ inflammation was induced, showing changes in collagen arrangement and properties using various imaging techniques, like staining and atomic force microscopy.
- Results indicated that inflammation led to a disordered collagen structure and mechanical weakness, highlighting how prolonged inflammation harms collagen integrity in TMJ discs.