Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The first non-natural derivative of the rare d-glucose-2-phosphate (G2P), namely glucose-2-(O-lactic acid phosphate) (G2LP), has been synthesized. When used as sole carbon source, G2LP enables bacterial growth of the plant pathogenic strain Agrobacterium fabrum C58 (formerly referred to as Agrobacterium tumefaciens). X-ray crystallography and affinity measurements investigations reveal that G2LP binds the periplasmic binding protein (PBP) AccA similarly to the natural compounds and with the same affinity. Moreover, enzymatic assays show that it is able to serve as substrate of the phosphodiesterase AccF. The properties found for G2LP demonstrate that the very unusual glucose-2-phosphoryl residue, present in G2LP, can be used as structural feature for designing non-natural systems fully compatible with the Acc cascade of A. fabrum.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c8ob03086c | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Acetylcholine chemotaxis in global bacterial plant pathogens.
Microbiol Res
November 2025
Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain. Electronic address:
Interactions within the plant holobiont involve a wide diversity of plant- and microbial-derived signals. Bacterial perception of these signals allows directed movement along chemical gradients through chemotaxis, a process that is an important determinant for plant colonization and infection. The quaternary amine acetylcholine is primarily known for its function as a neurotransmitter in mammalian nervous systems, but is emerging as a signal molecule that regulates plant growth, development, and stress resistance.
View Article and Find Full Text PDFThe efflux pump PecM is produced in response to the plant exudate 4-hydroxybenzaldehyde to avoid disruption of central metabolism.
J Bacteriol
July 2025
Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA.
is a phytopathogen that causes crown gall disease. In the rhizosphere, it encounters plant exudates, some of which are toxic, such as 4-hydroxybenzaldehyde (4HBA). Others, including 4-hydroxybenzoate (4HB), participate in the induction of virulence genes.
View Article and Find Full Text PDFA CRISPR-SpCas9M-reporting system for efficient and rapid genome editing in Caulobacter crescentus.
Nucleic Acids Res
April 2025
CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
As members of the α-proteobacteria group, Caulobacter crescentus and its relatives are wildly studied for their unique asymmetric life cycle and versatile applications in industry, agriculture, and biomedicine. However, genetic manipulation in these bacteria remains challenging, typically requiring time-consuming and labor-intensive procedures. Here, we report a practical CRISPR-SpCas9M-reporting system that overcomes the limitations of SpCas9 expression and CRISPR escape, enabling efficient, markerless, and rapid genome editing in C.
View Article and Find Full Text PDFThe Absence of a Very Long Chain Fatty Acid (VLCFA) in Lipid A Impairs Plant Infection and Biofilm Formation and Increases Susceptibility to Environmental Stressors.
Molecules
February 2025
Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Sklodowska University, 20-033 Lublin, Poland.
The C58 is a phytopathogen able to infect numerous species of cultivated and ornamental plants. During infection, bacteria genetically transform plant cells and induce the formation of tumours at the site of invasion. Bacterial cell wall components play a crucial role in the infection process.
View Article and Find Full Text PDFEnhancing wheat growth under chromium toxicity using gibberellic acid and microbial inoculants as modulating agents.
Sci Rep
March 2025
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia.
Chromium (Cr) is a highly toxic heavy metal that can negatively impact crop yield and food quality by causing chlorosis and reduced root and shoot growth. To address this issue, rhizobacteria has emerged as a viable and safe technology. Additionally, gibberellins (GA3) can act as allied factors for regulating various physiological processes in plants, particularly cell division and elongation under Cr stress.
View Article and Find Full Text PDF