Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/s41556-018-0251-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High-resolution imaging system for integration into intelligent noncontact total body scanner.
J Biomed Opt
September 2025
Leibniz University Hannover, Hannover Centre for Optical Technologies, Hannover, Germany.
Significance: Melanoma's rising incidence demands automatable high-throughput approaches for early detection such as total body scanners, integrated with computer-aided diagnosis. High-quality input data is necessary to improve diagnostic accuracy and reliability.
Aim: This work aims to develop a high-resolution optical skin imaging module and the software for acquiring and processing raw image data into high-resolution dermoscopic images using a focus stacking approach.
Advancements and perspectives on organelle-targeted fluorescent probes for super-resolution SIM imaging.
Chem Sci
September 2025
Institute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering, Guangxi University Nanning Guangxi 530004 China
As a cutting-edge super-resolution imaging technique, structured illumination microscopy (SIM) has been widely used in cell biology research, especially in the analysis of subcellular organelles and monitoring of their dynamic processes. Through multiple illumination and reconstruction processes, SIM breaks through the resolution limitations of traditional microscopes and can observe the fine structures within cells in real time with nanoscale resolution. This provides strong technical support for in-depth analyses of molecular mechanisms, organelle functions, signaling networks, and metabolic regulatory pathways within cells.
View Article and Find Full Text PDFOn-Target Photoassembly of Pyronin Dyes for Super-Resolution Microscopy.
Angew Chem Int Ed Engl
September 2025
Department of Optical Nanoscopy, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany.
Controlled photoactivation is an auspicious and emerging approach in super-resolution microscopy, offering virtually zero background signal from the marker prior to activation. Pyronins are well-established fluorophores, but due to their inherent intercalating tendency towards nucleic acids, their use has been mostly avoided in super-resolution microscopy. Here, we describe a new class of diaryl ether and diaryl silane molecules that upon photoactivation close into fluorescent (silicon-)pyronins and term them Pyronin Upon Light Irradiation (PULI).
View Article and Find Full Text PDFSuper-Resolution Compatible DNA Labeling Technique Reveals Chromatin Mobility and Organization Changes During Differentiation.
Adv Sci (Weinh)
September 2025
Cell Biology and Epigenetics, Department of Biology, Technical University of Darmstadt, 64287, Darmstadt, Germany.
Chromatin dynamics play a crucial role in cellular differentiation, yet tools for studying global chromatin mobility in living cells remain limited. Here, a novel probe is developeded for the metabolic labeling of chromatin and tracking its mobility during neural differentiation. The labeling system utilizes a newly developed silicon rhodamine-conjugated deoxycytidine triphosphate (dCTP).
View Article and Find Full Text PDFAttenuation of IFITM proteins' antiviral activity through sequestration into intraluminal vesicles of late endosomes.
Front Immunol
September 2025
Department of Pediatrics, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, GA, United States.
Introduction: Interferon-induced transmembrane proteins (IFITMs) inhibit the entry of diverse enveloped viruses. The spectrum of antiviral activity of IFITMs is largely determined by their subcellular localization. IFITM1 localizes to and primarily blocks viral fusion at the plasma membrane, while IFITM3 prevents viral fusion in late endosomes by accumulating in these compartments.
View Article and Find Full Text PDF