Induction of Functional Hepatocytes from Human iPSCs.

Methods Mol Biol

Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.

Published: June 2019


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Article Abstract

Human-induced pluripotent stem cells (iPSCs) could be a useful source for production of hepatocytes. Here, we develop protocols to generate iPSC-derived liver progenitor cells, liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs). We also establish long-term two-dimensional co-culture system to induce functional hepatocytes from iPSC-derived liver cells.

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http://dx.doi.org/10.1007/978-1-4939-8961-4_12DOI Listing

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