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Pectobacterium isolates SS95, SS54 and SS56 were collected from a potato field in the Chiniot district in the plains of the Punjab province, Pakistan. Sequencing of the gapA barcode revealed that these strains belong to a novel phylogenetic group separated from P.ectobacterium wasabiae and Pectobacterium parmentieri species. Furthermore, multilocus sequence analyses of 13 housekeeping genes (fusA, rpoD, acnA, purA, gyrB, recA, mdh, mtlD, groEL, secY, glyA, gapA and rplB) clearly distinguished the type strain, SS95, from its closest relatives, i.e. P. parmentieri RNS 08-42-1A and P. wasabiae CFBP3304, as well as from all the other known Pectobacteriumspecies. In silico DNA-DNA hybridization (<44.1 %) and average nucleotide identity (<90.75 %) values of strain SS95 compared with other Pectobacterium type strains supported the delineation of a new species. Genomic and phenotypic comparisons permitted the identification of additional traits that distinguished the Pakistani isolates from all other known Pectobacterium type strains. The name Pectobacterium punjabense sp. nov. is proposed for this taxon with the type strain SS95 (=CFBP 8604=LMG 30622).
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http://dx.doi.org/10.1099/ijsem.0.003029 | DOI Listing |
Plant Dis
September 2025
hainan universityhaikou, China, 570228;
Jackfruit (Artocarpus heterophyllus Lam.), a crucial economic crop in tropical regions, has recently been devastated by a novel gummosis disease in Hainan and Yunnan Provinces of China. This gummosis primarily affects the stems and branches of jackfruit, causing gum exudation, bark cracking, and plant death, which severely threatens the sustainable development of the industry.
View Article and Find Full Text PDFGenome Biol
August 2025
Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain.
Background: Chemotaxis to plant compounds is frequently the initial step for the colonization of plants by bacteria. Plant pathogens and plant-associated bacteria contain approximately twice as many chemoreceptors as the bacterial average does, indicating that chemotaxis is particularly important for bacteria-plant interactions. However, information on the corresponding chemoreceptors and their chemoeffectors is limited.
View Article and Find Full Text PDFMicroorganisms
August 2025
The Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, 1-1-1 Tsushima-naka, Kita-ku, Okayama 700-8530, Japan.
Genomic islands (GIs) including integrative and conjugative elements (ICEs), prophages, and integrative plasmids are central drivers of horizontal gene transfer in bacterial plant pathogens. These elements often carry cargo genes encoding virulence factors, antibiotic and metal resistance determinants, and metabolic functions that enhance environmental adaptability. In plant-pathogenic species such as , GIs contribute to host specificity, immune evasion, and the emergence of novel pathogenic variants.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Laboratory of NMR research of biosystems, Institute of Theoretical and Experimental Biophysics RAS, ul. Institutskaya 3, 142290 Pushchino, Russia.
A search for bacteriophage proteins with an EF-like calcium-binding loop, first discovered in the phage T5 endolysin (EndoT5), revealed a group of 71 peptidases of the M15_C subfamily. All the sequences found belonged to the phages infecting Gram-negative bacteria (Escherichia, Salmonella, Klebsiella, Shigella, Edwardsiella, Pectobacterium, Aeromonas, Shewanella etc.) In this work, we have characterized a hypothetical protein of Aeromonas bacteriophage 3, a representative of this group.
View Article and Find Full Text PDFPlant Dis
August 2025
Plant Health and Environment Laboratory, Ministry for Primary Industries, Auckland 1072, New Zealand.
is a significant kiwifruit canker pathogen reported from kiwifruit-growing areas in Asia and South America. To improve the diagnostic capability for this bacterium, a probe-based real-time PCR assay was developed for sensitive and fast detection of . The highly specific assay targets the meta-pathway phenol degradation protein-like locus, unique to .
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