Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Extracellular vesicles (EVs) have a great potential in clinical applications. However, their isolation from different bodily fluids and their characterisation are currently not optimal or standardised. Here, we report the results of examining the performance of ultrafiltration combined with size exclusion chromatography (UF-SEC) to isolate EVs from urine. The results reveal that UF-SEC is an efficient method and provides high purity. Furthermore, we introduce asymmetrical-flow field-flow fractionation coupled with a UV detector and multi-angle light-scattering detector (AF4/UV-MALS) as a characterisation method and compare it with current methods. We demonstrate that AF4/UV-MALS is a straightforward and reproducible method for determining size, amount and purity of isolated urinary EVs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032024 | PMC |
| http://dx.doi.org/10.1080/20013078.2018.1490143 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
"Sandwiched Individuals" Exploring the Push and Pull Factors Influencing Retention Among Mid-Career Nurses: An Integrative Review.
J Adv Nurs
September 2025
Nursing Department, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Aim: To systematically analyse international empirical literature and establish a comprehensive understanding of the push and pull factors influencing retention and turnover among mid-career nurses.
Design: An integrative review.
Data Sources: PubMed, Web of Science, Scopus, EMBASE (Ovid), and CINAHL (EBSCO) were searched for studies published between January 2001 and November 2024.
NMR-based structural integrity analysis of therapeutic monoclonal antibodies: a comparative study of Humira and its biosimilars.
MAbs
December 2025
Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.
The analytical comparability of biologic products and their biosimilars, including higher-order structure (HOS) assessment, ensures product quality and is required for regulatory approval. In this study, nuclear magnetic resonance (NMR) spectroscopy was used to evaluate the HOS of Humira (adalimumab) and its biosimilars under normal and photo-stressed conditions. Under normal conditions, 1D and 2D NMR spectra showed strong structural similarity among all products.
View Article and Find Full Text PDFGlobal prevalence of metabolic syndrome among individuals with bipolar disorder: A systematic review and meta-analysis.
Arch Psychiatr Nurs
October 2025
Research Center for Social Determinants of Health, Jahrom University of Medical Sciences, Jahrom, Iran. Electronic address:
Background: Metabolic syndrome is a widespread disease in the general population. The purpose of this study is to investigate the global prevalence of metabolic syndrome in the community of people with bipolar disorder through a systematic review and meta-analysis.
Methods: In this study, we conducted a systematic review and meta-analysis using electronic databases, including PubMed, Scopus, Web of Science, Embase, ScienceDirect, and the Google Scholar search engine.
Assessment of Amplitude and Temporal Differences of Cerebral Oxygen Saturation in Response to Hypotension Across Multiple Cerebrovascular Territories During Shoulder Surgery.
J Neurosurg Anesthesiol
October 2025
Department of Anesthesia and Perioperative Medicine, Western University.
Introduction: Current commercial cerebral oximeters only monitor the frontal lobes, however, some cerebrovascular territories may experience ischemia while others remain well perfused. This pilot study used a novel, high-density, dual-wavelength, time-resolved functional cerebral oximeter (Kernel Flow) with 2000 channels to assess the regional differences of cerebral oxygenation (StO2) in response to hypotension across different vascular territories during shoulder surgery in the beach chair position.
Methods: Twenty-seven adult patients were monitored, recording blood pressure, heart rate, regional cerebral oxygen saturation, and other vital parameters.
Epiduroscopy in spinal stenosis. Description of the procedure and safety measures to reduce complications.
Pain Manag
September 2025
Pain Management Unit, Hospital Universitario Quirónsalud Madrid, Madrid, Spain.
Aims: The aim of this observational study is to describe the use of epiduroscopy to decrease the enlargement of the ligamentum flavum (LF) in patients with spinal stenosis, as well as the selection of the appropriate patient and the safety measures that enhance procedural success.
Materials & Methods: We introduce the patient selection protocol, define the appropriate indication and the safety measures to use the epiduroscopy as a tool to decrease the size of the LF and increase space, reducing possible complications.
Results: Among patients included in the study, there were no cases of access difficulty or coccydynia, and one case of urinary incontinence occurred in a patient with Schizas grade D (very severe) stenosis.