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Acanthamoeba ocular infections, known as Acanthamoeba keratitis, are an emerging problem among contact lens wearers. Infections mediated by Acanthamoeba are uncommon, but they can be underestimated due to poor awareness and delayed diagnosis. The routine use of rapid and cost-effective molecular methods like Real Time PCR for the diagnosis of this important pathogen could improve diagnosis and therapy outcome. This report describes the detection by Real Time PCR assay of six T4 and one T3 Acanthamoeba infections, as the first reported cases in Tuscany, Italy.
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Exp Parasitol
September 2025
Department of Medical Parasitology, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt. Electronic address:
Background: Allovahlkampfia spelaea (A. spelaea) is a free-living amoeba that has recently been recognized to cause Acanthamoeba-like keratitis, the treatment of which is complex. The pathogenic potential of Allovahlkampfia spp.
View Article and Find Full Text PDFOphthalmology
September 2025
Francis I. Proctor Foundation, University of California San Francisco, USA; UCSF Department of Ophthalmology, University of California, San Francisco, USA; Byers Eye Institute, Stanford University, Palo Alto, USA. Electronic address:
Objective: To determine the effect of adjunctive rose-bengal photodynamic therapy (RB-PDT) in the treatment of fungal, Acanthamoeba, and smear/culture negative infectious keratitis.
Study Design: This international, randomized, double-masked, sham controlled clinical trial, randomizes patients with corneal ulcers in a 1:1 fashion to one of two treatment arms: 1) Topical antimicrobial plus sham RB-PDT or 2) Topical antimicrobial plus RB-PDT.
Main Outcome Measures: The primary outcome was best spectacle-corrected visual acuity (BSCVA) at 6 months.
Int J Microbiol
August 2025
Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran.
is a free-living amoeba that is widely found in nature in different environments such as soil, water, and dust. This parasite is the cause of amoebic keratitis and granulomatous amoebic encephalitis. This study is aimed at investigating the prevalence and genotypes of in different wards of Gonabad Bohlool Hospital, northeastern Iran.
View Article and Find Full Text PDFInt Ophthalmol
August 2025
Eye Hospital of Shandong First Medical University (Shandong Eye Hospital), Jinan, 250021, China.
Purpose: To compare the outcomes of corneal collagen cross-linking combined with lamellar keratoplasty (CXL-LK) versus LK alone in treating medically unresponsive acanthamoeba keratitis (AK).
Methods: This retrospective, non-randomized controlled clinical study included 11 eyes (CXL-LK group) and 16 eyes (LK group) at a tertiary ophthalmology center. Corneal infiltration, density of acanthamoeba cysts (AC) and pathological changes following CXL in AK patients were assessed.
Parasitol Res
August 2025
Institute of Pharmaceutical Technology and Biopharmaceutics, Technische Universität Braunschweig, Brunswick, Germany.
Corneal exposure to trophozoites of Acanthamoeba spp. may lead to Acanthamoeba keratitis (AK)-a rare, but sight-threatening disease-with a risk of recurrence due to residual stromal cysts. With polyhexanide (PHMB) and chlorhexidine (CHX) often constituting the standard regimen of therapy, polymeric compounds for the treatment of AK have shifted into the focus of research.
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