Response surface methodology as a tool for modelling galacto-oligosaccharide production.

J Dairy Res

Instituto de Lactología Industrial-Universidad Nacional del Litoral/Consejo Nacional de Investigaciones Científicas y Técnicas (INLAIN-UNL/CONICET),Santiago del Estero 2829, S3000AOM Santa Fe,Argentina.

Published: November 2017


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time. The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM). The results indicate that the proposed mathematical models could adequately describe the concentration and yield of GOS and the reaction time within the limits of the factors that are being investigated. The variance analysis shows high values of coefficients of determination (>0·97) while no significant lack of fit was evident. Hence, the models could be employed to select reaction conditions applied in the manufacture of products enriched in bioactive compounds with high value-added.

Download full-text PDF

Source
http://dx.doi.org/10.1017/S0022029917000541DOI Listing

Publication Analysis

Top Keywords

response surface
8
surface methodology
8
concentration yield
8
gos reaction
8
reaction time
8
methodology tool
4
tool modelling
4
modelling galacto-oligosaccharide
4
galacto-oligosaccharide production
4
production experiments
4

Similar Publications

Transcranial alternating current stimulation (tACS) enables non-invasive modulation of brain activity, holding promise for cognitive research and clinical applications. However, it remains unclear how the spiking activity of cortical neurons is modulated by specific electric field (E-field) distributions. Here, we use a multi-scale computational framework that integrates an anatomically accurate head model with morphologically realistic neuron models to simulate the responses of layer 5 pyramidal cells (L5 PCs) to the E-fields generated by conventional M1-SO tACS.

View Article and Find Full Text PDF

Energy production from renewable resources remains a leading focus in sustainable power generation. Recently, bifacial photovoltaic (BPV) systems have gained global attention for their enhanced energy yield. In this study, seashell waste was repurposed as an alternative reflector material for BPV modules.

View Article and Find Full Text PDF

Carbon quantum dot-aptamer/MoS nanosheet fluorescent sensor for ultrasensitive, noninvasive cortisol detection.

Anal Bioanal Chem

September 2025

Hebei Key Laboratory of Public Health Safety, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Public Health, College of Chemistry and Materials Science, Hebei University, Baoding, 071002, China.

This work presents the development of a highly sensitive, selective, and efficient aptamer-based fluorescent sensor for detecting cortisol in human urine. Carbon quantum dots-nucleic acid aptamer (CQDs-Apt) synthesized with excellent photoluminescent properties and stability, were selected as the fluorescent probe. In the presence of MoS-NSs, CQDs-Apt adsorbed onto the surface of MoS-NSs via electrostatic and π-π interactions, leading to strong and rapid fluorescence quenching due to static quenching mechanism between them.

View Article and Find Full Text PDF

Distinctive polymer brushes (PBs) play a crucial role in providing a nonpreferential (neutral) surface for vertical orientation of block copolymers (BCPs). This bottom-up approach effectively aligns the formation of vertical lamellar and cylinder lattice structures from the BCP, which is crucial for nanopatterning and other applications. In conventional BCP self-assembly techniques, random copolymer brushes are commonly employed to achieve substrate neutrality.

View Article and Find Full Text PDF

Biofilm lifestyle across different lineages of ammonia-oxidizing archaea.

ISME J

September 2025

Department of Functional and Evolutionary Ecology, Archaea Biology and Ecogenomics Unit, University of Vienna, Djerassiplatz 1, 1030 Vienna, Austria.

Although ammonia-oxidizing archaea (AOA) are globally distributed in nature, growth in biofilms has been relatively little explored. Here we investigated six representatives of three different terrestrial and marine clades of AOA in a longitudinal and quantitative study for their ability to form biofilm, and studied gene expression patterns of three representatives. Although all strains grew on a solid surface, soil strains of the genera Nitrosocosmicus and Nitrososphaera exhibited the highest capacity for biofilm formation.

View Article and Find Full Text PDF