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Article Abstract
Unlabelled: N6.2 mitigates the onset of type 1 diabetes (T1D) in biobreeding diabetes-prone rats, in part, through changes in kynurenine:tryptophan (K:T) ratios. The goal of this pilot study was to determine the safety, tolerance, and general immunological response of N6.2 in healthy subjects. A double-blind, randomized clinical trial in 42 healthy individuals with no known risk factors for T1D was undertaken to evaluate subject responses to the consumption of N6.2. Participants received 1 capsule/day containing 10 colony-forming units of N6.2 or placebo for 8 weeks. Comprehensive metabolic panel (CMP), leukocyte subpopulations by complete blood count (CBC) and flow cytometry, serum cytokines, and relevant metabolites in the indoleamine-2,3-dioxygenase pathway were assessed. N6.2 survival and intestinal microbiota was analyzed. Daily and weekly questionnaires were assessed for potential effects of probiotic treatment on general wellness. The administration of N6.2 did not modify the CMP or CBC of participants suggesting general safety. In fact, N6.2 administration significantly decreased the occurrence of abdominal pain, indigestion, and cephalic syndromes. As predicted, increased serum tryptophan levels increased resulting in a decreased K:T ratio was observed in the N6.2 group. Interestingly, immunophenotyping assays revealed that monocytes and natural killer cell numbers were increased significantly after washout (12 weeks). Moreover, an increase of circulating effector Th1 cells (CD45ROCD183CD196) and cytotoxic CD8 T cells subset was observed in the N6.2 group. Consumption of N6.2 is well tolerated in adult control subjects, demonstrates systemic impacts on innate and adaptive immune populations, and results in a decreased K:T ratio. These data provide support for the safety and feasibility of using N6.2 in prevention trials in subjects at risk for T1D.
Trial Registration: This trial was registered at http://clinicaltrials.gov as NCT02349360.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466969 | PMC |
| http://dx.doi.org/10.3389/fimmu.2017.00655 | DOI Listing |
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