Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Babesia and Theileria species are tick-borne protozoan parasites that have a veterinary and zoonotic importance. In order to investigate the prevalence and genetic diversity of these parasites, a total of 269 sika deer blood DNA samples collected from Hokkaido, Japan, were examined for Babesia and Theileria species by touch-down PCR targeting the 18S rRNA gene. Reverse line blot (RLB) hybridization was then used to detect 12 piroplasm species. The results revealed that 95.5% (257/269), 94.1% (253/269), 14.1% (38/269), 87.7% (236/269) and 11.5% (31/269) of the examined PCR products hybridized with the probes which were designed to detect all Babesia and Theileria spp., all Theileria spp., all Babesia spp., Theileria sp. Thrivae and Babesia divergens-like, respectively. The 18S rRNA gene partial sequences were divided into Theileria sp. Thrivae, T. capreoli, B. divergens-like and an undescribed Babesia species. This study showed the first detection of the undescribed Babesia sp. from Japan. Therefore, more studies are required to understand the ecology of the newly detected tick-borne pathogens in Hokkaido.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ttbdis.2017.06.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
in (Acari: Ixodida, Ixodidae) collected on horses from North-western Argentina.
J Parasit Dis
September 2025
Instituto de Investigación de la Cadena Láctea (IdICaL), CONICET - INTA, RN No 34, KM 227, CP-2300 Rafaela, Santa Fe Argentina.
ticks collected on horses from the North-West of Argentina were analyzed for the presence of the Piroplasm agents and . A total of 97 adult ticks from four different provinces (Jujuy, Santiago del Estero, Salta, Tucumán) were included in this study. Genomic DNA was isolated, and conventional PCR assays were applied for the detection of the 18S rDNA gene of and species.
View Article and Find Full Text PDFWidespread infections with bacterial and protozoan tick-borne pathogens in 22 wildlife species across South African game reserves.
Acta Trop
August 2025
Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa.
Tick-borne pathogens (TBPs) are a growing global concern, contributing to emerging and re-emerging diseases in humans and animals. Human encroachment into natural habitats and unregulated wildlife translocations are key drivers of TBP emergence, as they expand wildlife-livestock-human interfaces and facilitate the introduction of alien TBPs into naïve hosts and new regions. Accurate molecular surveillance is essential to guide management strategies and prevent clinical outbreaks.
View Article and Find Full Text PDFSeasonal Dynamics and Pathogen Diversity of Tick Species Parasitizing Migratory Birds in Sardinia, Italy: Implications for the Spread of , , and Species.
Vet Sci
August 2025
Istituto Zooprofilattico Sperimentale della Sardegna "G. Pegreffi", Via Duca degli Abruzzi 8, 07100 Sassari, Italy.
Migratory birds play a key role in the ecology of tick-borne pathogens, serving as both hosts for ticks and as potential carriers of a wide range of infectious agents that can affect wildlife, domestic animals, and humans. Their long-distance movements contribute to the dispersal of ticks and the pathogens they harbor, with potential implications for the emergence and spread of zoonotic disease. This study focuses on the prevalence of spp.
View Article and Find Full Text PDFDevelopment and validation of a real-time SYBR green PCR method for the detection and differentiation of Babesia and Theileria species (Apicomplexa: Piroplasmida) in hard ticks and cattle blood from Thailand.
Parasite
August 2025
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand - Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, 40002, Thailand.
Tick-borne pathogens, particularly Babesia and Theileria species, are major threats to cattle production, causing economically significant diseases such as babesiosis and theileriosis. In this study, a real-time SYBR Green PCR assay was developed to detect Babesia and Theileria species in hard ticks (N = 65) and cattle blood samples (N = 143) from Thailand. Using primers targeting the mitochondrial cytochrome b gene for Babesia and the nuclear 18S rRNA gene for Theileria, the assay measured specific melting temperatures (Tm) for each species.
View Article and Find Full Text PDFTheileria luwenshuni and Novel Babesia spp. Infections in Humans, Yunnan Province, China.
Emerg Infect Dis
September 2025
Piroplasmid parasites such as Theileria luwenshuni protozoa pose a global threat to both animal and human health. However, human theileriosis remains underexplored compared to infections caused by Plasmodium and Babesia species parasites. We investigated potential hemoparasite infections among 1,721 persons with fever, anemia, or both in Yunnan Province, China.
View Article and Find Full Text PDF