[Study on the Characteristics of Bone in Type-2 Diabetic Rats by Micro-CT].

Objectives: To study the characteristics of bone in type 2 diabetic rats using micro-CT.

Methods: Sixteen male Sprague Dawley(SD) rats, aged 8 week-old, were randomly divided into normal group and experimental group ( =10). Afer being fed with high-fat chow, the rats in experimental group were given low-dose streptozotocin (STZ,30 mg/kg) to induce type 2 diabetic disease. Serum glucose, insulin and tissue sections of pancreas is used to evaluate the effect of type 2 diabetic rat model. The characteristics of bone in the two groups were measured and compared by micro-CT.

Results: Compared with normal group ( =10), the experimental group ( =9) had the body mass and serum glucose increased significantly (<0.05). The level of serum insulin was equivalent in two groups(>0.05). Pancreatic tissue section staining showed in experimental group islet morphology was irregular, edge boundary was blur and islet cells were saperated with increasing fiber tissue. The bone volume of normal and experimental groups were (0.194 2±2.332)%, and (0.080 7±1.952)%, respectively. Trabecular thickness (unit:mm) were 0.052 9±0.004 5 and 0.043 6±0.002 4, respectively. Trabecular number (unit:mm⁻¹) were 3.668 8±0.134 5, and 1.851 7±0.288 8, respectively ( <0.05), while trabecular space (unit:mm) were 0.219 6±0.072 1 and 0.496 5±0.076 4, respectively( <0.05). Bone tissue morphology metrology test results was consistent with the results of micro-CT.

Conclusions: The diabetic rat model showed declined bone volume and trabecular density, trabecular number. Micro-CT may be more intuitive in bone quality inspection to reflect the characteristics of bone microstructure morphology.