Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Aim: In this study, we aimed to analyze the relationship of phosphorus-rich structures with surface architecture in Cryptococcus neoformans.
Methods: Phosphorus-rich structures in C. neoformans were analyzed by combining fluorescence microscopy, biochemical extraction, scanning electron microscopy, electron probe x-ray microanalysis and 3D reconstruction of high pressure frozen and freeze substituted cells by focused ion beam-scanning electron microscopy (FIB-SEM).
Results & Conclusion: Intracellular and surface phosphorus-enriched structures were identified. These molecules were required for capsule assembly, as demonstrated in experiments using polysaccharide incorporation by capsule-deficient cells and mutants with defects in polyphosphate synthesis. The demonstration of intracellular and cell wall-associated polyphosphates in C. neoformans may lead to future studies involving their participation in both physiologic and pathogenic events.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2217/fmb-2017-0060 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Tubotomaculum Enigma and the Rise of Benthic Life During the Opening of the Western Mediterranean Basin.
Geobiology
September 2025
Dipartimento di Scienze, Università di Roma Tre, Roma, Italy.
Large-scale geological processes shape microbial habitats and drive the evolution of life on Earth. During the Oligocene, convergence between Africa and Europe led to the opening of the Western Mediterranean Basin, a deep-ocean system characterized by fluid venting, oxygen depletion, and the absence of benthic fauna. In this extreme, inhospitable seafloor environment, fusiform objects known as Tubotomaculum formed, whose origin has long remained controversial.
View Article and Find Full Text PDFInfluence of Annealing on the Properties of FeNiPC Alloy.
Materials (Basel)
July 2025
Faculty of Mechanical Engineering, Silesian University of Technology, ul. Akademicka 2A, 44-100 Gliwice, Poland.
In this study, the influence of annealing on the phase evolution and mechanical properties of the FeNiPC (at.%) alloy was investigated. Ribbons produced via melt-spinning were annealed at various temperatures, and their structural transformations and hardness were evaluated.
View Article and Find Full Text PDFEnrichment process of phosphorite type REY based on the structure and geochemical features of the Zhijin Xinhua phosphorite in China.
Sci Rep
July 2025
School of Resource and Environmental Engineering, Guizhou University, Guiyang, 500025, China.
In response to the insufficiently in-depth and systematic studies on the enrichment processes of phosphorite-type rare earths (REE), this study takes the recently discovered Early Cambrian Xinhua rare earth-phosphate composite deposit in Zhijin, Guizhou, as a case example. Building on detailed field investigations, this study conducts an in-depth analysis of the ore's structures. Employing a suite of analytical techniques, including X-Ray Fluorescence (XRF), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Total Organic Carbon (TOC) testing, and Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS), we meticulously delineate the sourcing, transportation, and storage processes of REE.
View Article and Find Full Text PDFHigh Dietary Phosphorus Impairs Bone Microarchitecture and Induces Alterations in the LGR4-R-Spondins Axis in Rats with Normal Renal Function.
Nutrients
June 2025
Metabolismo Óseo, Vascular y Enfermedades Inflamatorias Crónicas, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain.
The increasing prevalence of processed foods has significantly elevated dietary phosphorus intake globally, posing a risk to skeletal health. Elevated serum phosphate promotes parathyroid hormone (PTH) release, leading to bone resorption and decreased bone formation. : This study investigated the influence of chronically elevated phosphorus intake on bone structure in rats with normal renal function, focusing on the Receptor Activator of Nuclear factor Kappa-B (RANK)/RANK ligand (RANKL)/osteoprotegerin (OPG) pathway and its related components, leucine rich repeat containing G protein-coupled receptor 4 (LGR4), and R-spondins (RSPOs).
View Article and Find Full Text PDFNanobeam-scanning X-ray fluorescence microscopy reveals the elemental composition of dense intracellular bodies in biomineralizing coccolithophores.
Faraday Discuss
June 2025
University of Greifswald, Institute of Microbiology, Felix-Hausdorff-Straße 8, 17489 Greifswald, Germany.
Coccolithophore microalgae intracellularly produce nanostructured calcitic platelets, known as coccoliths, through a biologically-controlled mineralization process. Mature coccoliths are secreted to the cell surface and assembled into a shell that envelops the cell. The large-scale global production of coccoliths, followed by their sedimentation to the ocean floor, significantly contributes to carbon cycling.
View Article and Find Full Text PDF