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The generalized concept of 'dynamic' buffer capacity is related to electrolytic systems of different complexity where acid-base equilibria are involved. The resulting formulas are presented in a uniform and consistent form. The detailed calculations are related to two Britton-Robinson buffers, taken as examples.
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http://dx.doi.org/10.1007/s10953-015-0342-0 | DOI Listing |
J Nutr Biochem
September 2025
Multicentric Postgraduate Program in Physiological Sciences, SBFis, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil; Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil; Postgraduate Program in Sciences, Pedi
Studies indicate that dietary patterns influence the function and redox balance of salivary glands. This study examined the effects of intermittent fasting (IF) on the function, histological structure, and redox balance of the salivary glands. Twenty 12-weeks-old male Wistar rats were randomized into two groups: ad libitum (AL), with continuous access to water and chow, and IF, subjected to 24-hour fasting on alternate days for 12 weeks.
View Article and Find Full Text PDFA key goal of vaccinology is to train the immune system to combat current pathogens while simultaneously preparing it for future evolved variants. Understanding factors contributing to anticipatory breadth, wherein affinity maturation against an ancestral strain yields neutralization capacity against evolved variants, is therefore of great importance. Here, we investigated the mechanism of anticipatory breadth development in a public antibody family targeting the functionally restricted ACE2 binding site on SARS-CoV-2.
View Article and Find Full Text PDFJ Chromatogr A
August 2025
Waters Corporation, Core Research/Fundamental Milford, MA, 01757, USA.
Slalom chromatography (SC) has recently been revitalized through the latest available UHPLC columns and systems, alongside advances in better understanding its separation and mass transfer mechanisms. These developments have demonstrated its potential in the analysis of cell and gene therapy drug substances, including plasmid topology analysis, DNA restriction mapping, dsRNA impurity detection in IVT mRNA, and CRISPR-Cas9 ribonucleoprotein assays. However, SC's quantitative superiority over traditional agarose gel electrophoresis (AGE) remains unproven, and no accurate and predictive model has yet been reported for any commercial SC column for a wide range of experimental conditions of temperature, flow rate, and buffer concentration.
View Article and Find Full Text PDFFood Chem
August 2025
Food Chemistry, Department of Chemistry and Pharmacy, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Nikolaus-Fiebiger-Str. 10, 91058 Erlangen, Germany; FAU NeW - Research Center New Bioactive Compounds, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Nikolaus-Fiebiger-Str. 10
Thermal processing of whey-based foods leads to nonenzymatic post-translational protein modifications (nePTMs), which reduce the protein quality. Because pyridoxamine inhibits protein glycation and oxidation in vivo and in model reactions, the present study investigated the effect of pyridoxamine on β-lactoglobulin modifications in heated whey. After Glu-C digestion, 14 nePTMs were quantified at 29 binding sites by micro-liquid chromatography-electrospray ionization-tandem mass spectrometry in scheduled multiple reaction monitoring mode.
View Article and Find Full Text PDFSmall Methods
September 2025
State Key Laboratory of Material Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.
Electrocatalytic reactions play a pivotal role in advancing sustainable energy technologies, particularly in the conversion of renewable resources into clean fuels. Achieving high-efficiency and durable electrocatalysts is essential for overcoming kinetic barriers in key processes. In this context, fullerenes have emerged as promising building blocks for catalyst design, owing to their unique structural and electronic characteristics.
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