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Amoebae of the genus Acanthamoeba occur worldwide and in addition to being pathogens, are important vehicles for microorganisms with clinical and environmental importance. This study aimed to evaluate the profiling of endosymbionts in 12 isolates of Acanthamoeba using V3 region of 16S rDNA denaturing gradient gel electrophoresis (DGGE) and sequencing. The DGGE enabled us to characterize the endosymbionts diversity in isolates of Acanthamoeba, and to identify Paenibacillus sp., an emerging pathogen, as an amoebic endosymbiont. The results of this study demonstrated that Acanthamoeba is capable of transporting a large number of endosymbionts. This is the first study that reports, the presence of Paenibacillus sp. as amebic symbiont.
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http://dx.doi.org/10.1007/s00284-015-0869-8 | DOI Listing |
Exp Parasitol
September 2025
Department of Medical Parasitology, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt. Electronic address:
Background: Allovahlkampfia spelaea (A. spelaea) is a free-living amoeba that has recently been recognized to cause Acanthamoeba-like keratitis, the treatment of which is complex. The pathogenic potential of Allovahlkampfia spp.
View Article and Find Full Text PDFOphthalmology
September 2025
Francis I. Proctor Foundation, University of California San Francisco, USA; UCSF Department of Ophthalmology, University of California, San Francisco, USA; Byers Eye Institute, Stanford University, Palo Alto, USA. Electronic address:
Objective: To determine the effect of adjunctive rose-bengal photodynamic therapy (RB-PDT) in the treatment of fungal, Acanthamoeba, and smear/culture negative infectious keratitis.
Study Design: This international, randomized, double-masked, sham controlled clinical trial, randomizes patients with corneal ulcers in a 1:1 fashion to one of two treatment arms: 1) Topical antimicrobial plus sham RB-PDT or 2) Topical antimicrobial plus RB-PDT.
Main Outcome Measures: The primary outcome was best spectacle-corrected visual acuity (BSCVA) at 6 months.
Invest Ophthalmol Vis Sci
July 2025
Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Purpose: Acanthamoeba keratitis (AK) is a severe infection linked to orthokeratology lens use, whereas the involvement of conjunctival microbiota in AK remains poorly understood. This study investigates microbiota dysbiosis in AK pathogenesis to inform microbiota-based interventions.
Methods: Conjunctival swabs from 14 patients with AK and 10 healthy controls underwent 16S rRNA sequencing.