Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Cytoplasmic dynein 1 (dynein) is a minus end-directed microtubule motor protein with many cellular functions, including during cell division. The role of the light intermediate chains (LICs; DYNC1LI1 and 2) within the complex is poorly understood. In this paper, we have used small interfering RNAs or morpholino oligonucleotides to deplete the LICs in human cell lines and Xenopus laevis early embryos to dissect the LICs' role in cell division. We show that although dynein lacking LICs drives microtubule gliding at normal rates, the LICs are required for the formation and maintenance of a bipolar spindle. Multipolar spindles with poles that contain single centrioles were formed in cells lacking LICs, indicating that they are needed for maintaining centrosome integrity. The formation of multipolar spindles via centrosome splitting after LIC depletion could be rescued by inhibiting Eg5. This suggests a novel role for the dynein complex, counteracted by Eg5, in the maintenance of centriole cohesion during mitosis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4242835 | PMC |
| http://dx.doi.org/10.1083/jcb.201408025 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Detection of a hybrid PrPfr state in the dark reversion of a bathy phytochrome indicates inter-dimer allostery.
Phys Chem Chem Phys
September 2025
Department of Chemistry - BMC, Biochemistry, Uppsala University, 75123 Uppsala, Sweden.
Phytochromes are photosensor proteins found in plants, fungi, and bacteria. They photoswitch between red light absorbing (Pr) and far-red light absorbing (Pfr) states. Thermal reversion in the dark, however, is an equally important factor in controlling their signaling levels.
View Article and Find Full Text PDFContinuous Flow Photocatalysis Boosting C─N Coupling for Sustainable High-Efficiency Formamide Synthesis.
Angew Chem Int Ed Engl
September 2025
Hefei National Research Center for Physical Sciences at the Microscale, School of Chemistry and Materials Science, State Key Laboratory of Precision and Intelligent Chemistry, National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, 230026, China.
The construction of C─N bonds from simple precursors under ambient conditions is a fundamental challenge in green chemistry, especially when it comes to avoiding energy-intensive protocols. Here, we present a continuous flow photocatalytic platform that enables the efficient coupling of C─N bonds between methanol and ammonia at ambient temperature and pressure. By synergistically engineering a Pd clusters-decorated TiO photocatalyst (1Pd/TiO) and a mass transfer-enhanced gas-liquid-solid Taylor flow reactor, the system achieves a remarkable formamide productivity of 256.
View Article and Find Full Text PDFQuinoline as a Photochemical Toolbox: From Substrate to Catalyst and Beyond.
Acc Chem Res
September 2025
Department of Chemistry, FRQNT Centre for Green Chemistry and Catalysis, McGill University, 801 Sherbrooke Street W, Montréal, Québec H3A 0B8, Canada.
ConspectusMolecular photochemistry, by harnessing the excited states of organic molecules, provides a platform fundamentally distinct from thermochemistry for generating reactive open-shell or spin-active species under mild conditions. Among its diverse applications, the resurgence of the Minisci-type reaction, a transformation historically reliant on thermally initiated radical conditions, has been fueled by modern photochemical strategies with improved efficiency and selectivity. Consequently, the photochemical Minisci-type reaction ranks among the most enabling methods for C()-H functionalizations of heteroarenes, which are of particular significance in medicinal chemistry for the rapid diversification of bioactive scaffolds.
View Article and Find Full Text PDFComputational Design of B,N-Substituted Graphene Ribbons Exhibiting S and T States with Near-Degeneracy and High Oscillator Strengths.
Chemphyschem
September 2025
Institute of Physics, Polish Academy of Sciences, PL-02-668, Warsaw, Poland.
B,N-substituted graphene ribbons are computationally designed and their spectroscopic properties are systematically explored with wave-function-based electronic structure methods. All B,N-graphene ribbons exhibit exceptionally small S-T energy gaps. The oscillator strength of the S-S transition increases monotonically with the length of the ribbons.
View Article and Find Full Text PDFUnveiling the Hierarchical Network of Sleep Quality Determinants: Linking Behavioral, Environmental, and Psychosocial Pathways.
Psychol Res Behav Manag
September 2025
Department of Internal Medicine, Shaoxing Second Hospital, Shaoxing City, Zhejiang Province, People's Republic of China.
Background: Sleep quality has emerged as a critical public health concern, yet our understanding of how multiple determinants interact to influence sleep outcomes remains limited. This study employed partial correlation network analysis to examine the hierarchical structure of sleep quality determinants among Chinese adults.
Methods: We investigated the interrelationships among nine key factors: daily activity rhythm, social interaction frequency, work-life balance, light exposure, physical activity level, time control perception, shift work, weekend catch-up sleep, and sleep quality using the extended Bayesian Information Criterion (EBIC) glasso model.