Changes in cellular thiol content and intracellular Zn(2+) level by 1,4-naphthoquinone in rat thymocytes.

1,4-Naphthoquinone is an active metabolite of naphthalene and it is also found in diesel exhaust particles. It is known to cause oxidative stress. In this study, we characterized 1,4-naphthoquinone-mediated cytotoxicity and its effects on the levels of non-protein thiols and intracellular Zn(2+) in rat thymocytes (thymic lymphocytes) by using 5-chloromethylfluorescein (5-CMF) fluorescence and FluoZin-3 fluorescence, respectively. Low concentrations of 1,4-naphthoquinone (0.3μM) increased the intensity of 5-CMF fluorescence, which is used to measure non-protein thiols. In contrast, 5-CMF intensity decreased at higher concentrations (1-3μM) of 1,4-naphthoquinone. Removal of intracellular Zn(2+) attenuated the 1,4-naphthoquinone-induced augmentation of 5-CMF fluorescence. Additionally, 1,4-naphthoquinone (0.3-3μM) increased FluoZin-3 fluorescence, which is used to assess intracellular Zn(2+), in a concentration-dependent manner. The augmentation of FluoZin-3 fluorescence by 1,4-naphthoquinone was due to the release of intracellular Zn(2+), because the removal of extracellular Zn(2+) did not affect the augmentation of FluoZin-3 fluorescence. These results suggest that sublethal concentrations of 1,4-naphthoquinone (0.3-1μM) affect the cellular levels of non-protein thiols and intracellular Zn(2+). The difference in the observed decrease in cellular thiol content due to 1,4-naphthoquinone treatment and increase due to Zn(2+) release following 1,4-naphthoquinone treatment likely confers the change in cellular thiol content. Further, the increase in intracellular Zn(2+) concentration after 1,4-naphthoquinone exposure may change the activity of thymocytes because thymulin, a thymus-specific hormone, requires Zn(2+) for its biological activity.