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By anchoring 1,2,4,5-tetrazine-containing biomolecules onto trans-cyclooctene (TCO)-functionalized slides, a site-specific microarray immobilization approach is described in this study. Compared with existing immobilization methods, our approach offers several distinctive features, including fast kinetics and high chemoselectivity.
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http://dx.doi.org/10.1039/c4cc03838j | DOI Listing |
Analyst
September 2025
School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.
Microfluidics-assisted spatially barcoded microarray technology offers a high-throughput, low-cost approach towards spatial transcriptomic profiling. A uniform barcoded microarray is crucial for spatially unbiased mRNA analysis. However, non-specific adsorption of barcoding reagents in microchannels occurs during liquid transport, causing non-uniform barcoding in the chip's functional regions.
View Article and Find Full Text PDFMol Biol (Mosk)
August 2025
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
Microarrays with immobilized protein probes are used to analyze protein samples. Selection of materials for biochip fabrication, functionalization of the carrier surface, construction of ordered cell matrices, immobilization of protein molecular probes in cells, and improvement of the sensitivity of protein sample analysis are key tasks of biochip technology. The following methodological approaches were used in this work.
View Article and Find Full Text PDFN-phosphorylation is a ubiquitous post-translational modification that plays a pivotal role in regulating protein activity, function, interactions, and subcellular localization. However, its functional roles in biological systems remain largely unexplored, due to the absence of N-phospho-specific antibodies and the inherent instability of the phosphoramidate (P-N) bond. To address this challenge, we introduce the reverse-phase N-phosphoprotein array (RPNPA) as a novel and efficient solution.
View Article and Find Full Text PDFSmall Methods
August 2025
Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.
Influenza viruses use haemagglutinins (HA) to target host sialic acids in the respiratory tract as do other pathogens, including coronaviruses, which engage using spike protein. The host adaptation of the HA protein, which leads to the accumulation of mutations, is a key descriptor of individual influenza strains, which aids zoonosis and is crucial in vaccine development. How each strain targets glycans is crucial to understanding function, designing new therapies, and optimizing candidates for vaccine development.
View Article and Find Full Text PDFMethods Mol Biol
July 2025
Wageningen Bioveterinary Research, Lelystad, The Netherlands.
Bead-based suspension arrays built with multiple antigens enable detection and identification of specific antibodies in human and animal serum. Here we describe how to use synthetic peptides as antigens in a serological Luminex assay. Biotinylated peptides immobilized on avidin beads are used to capture serum antibodies, which in turn are detected using a generic immunoglobulin-binding protein or broad anti-bird antibodies in resp.
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