Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Outbreak of WSSV disease is one of the major stumbling blocks in shrimp aquaculture. DNA vaccines have shown potential for mass scale vaccination owing to their stability, cost effectiveness and easy maintenance. Development of economically feasible delivery strategies remains to be a major challenge. This study demonstrates vertical transmission of a plasmid DNA in a decapod Macrobrachium rosenbergii for the first time. Females at three different maturation stages (immature, matured and berried) and mature males were injected with a plasmid DNA and allowed to spawn with untreated counterparts. Using specific primers the plasmid DNA could be amplified from the offspring of all groups except that of berried females. For this confirmation genomic DNA was isolated from 3 pools of 10 post larvae in each group. This presents an ideal strategy to protect young ones at zero stress.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jip.2014.06.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the effect of short-term exposure to non-functionalized polystyrene nanoparticles on selected chromatin determinants in human immune cells and plasmid DNA integrity.
Nanotoxicology
September 2025
Department of Biophysics of Environmental Pollution, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
The effect of non-functionalized polystyrene nanoparticles (PS-NPs) with diameters of 29, 44, and 72 nm on plasmid DNA integrity and the expression of genes involved in the architecture of chromatin was investigated in human peripheral blood mononuclear cells (PBMCs). The cells were incubated with PS-NPs at concentrations ranging from 0.001 to 100 µg/mL for 24 hours.
View Article and Find Full Text PDFH2A.Z-nucleosomes are stabilized by the superhelicity-dependent DNA binding of the C-terminal tail of the histone variant.
Nucleus
December 2025
Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Using an in situ nucleosome stability assay based on salt extraction, we identified distinct stability features of H2A.Z-containing nucleosomes linked to alternative interactions of the histone variant's C-terminal tail (Imre et al., Nat.
View Article and Find Full Text PDFTitanium dioxide nanoparticles as a promising tool for efficient separation of trace DNA via phosphate-mediated desorption.
Mikrochim Acta
September 2025
Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, 28 Changsheng West Road, Hengyang, 421001, Hunan, China.
We systematically evaluated the DNA adsorption and desorption efficiencies of several nanoparticles. Among them, titanium dioxide (TiO₂) nanoparticles (NPs), aluminum oxide (Al₂O₃) NPs, and zinc oxide (ZnO) NPs exhibited strong DNA-binding capacities under mild conditions. However, phosphate-mediated DNA displacement efficiencies varied considerably, with only TiO₂ NPs showing consistently superior performance.
View Article and Find Full Text PDFEscherichia coli strain O55 contains two cryptic plasmids that depend on each other to replicate.
Arch Microbiol
September 2025
División de Ciencias Naturales y Exactas, Departamento de Biología, Universidad de Guanajuato, Zip Code 36050, Guanajuato, Mexico.
Plasmids are fundamental to molecular biology and biotechnology, playing a crucial role in bacterial evolution. Some plasmids are linked to complex cellular dynamics, including pathogenicity islands, antibiotic resistance, and gene mobilization. This study reports the isolation and sequencing of two cryptic plasmids with different electrophoretic mobilities from the Escherichia coli clinical isolate O55.
View Article and Find Full Text PDFLactococcal mobile genetic elements harbour a diverse phage defensome rich in restriction-modification systems.
Nucleic Acids Res
September 2025
School of Microbiology, University College Cork, Cork, T12 Y337, Ireland.
The genomes of 43 distinct lactococcal strains were reconstructed by a combination of long- and short-read sequencing, resolving the plasmid complement and methylome of these strains. The genomes comprised 43 chromosomes of approximately 2.5 Mb each and 269 plasmids ranging from 2 to 211 kb (at an average occurrence of 6 per strain).
View Article and Find Full Text PDF