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A scalable approach for developing large area polymer films, with stimuli responsive vertically aligned nanopores is reported. Magnetic fields are used to create highly aligned hexagonally packed block copolymer cylindrical microdomains with order parameters exceeding 0.95. Selective etch removal of material yields nanoporous films which demonstrate reversible pore closure on heating.
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http://dx.doi.org/10.1002/adma.201401569 | DOI Listing |
Eur J Haematol
September 2025
Haematology-Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.
Background: Clonotyping of immunoglobulin heavy chain (IGH) gene rearrangements is critical for diagnosis, prognostication, and measurable residual disease monitoring in chronic lymphocytic leukemia (CLL). Although short-read next-generation sequencing (NGS) platforms, such as Illumina MiSeq, are widely used, they face challenges in spanning full VDJ rearrangements. Long-read sequencing via Oxford Nanopore Technologies (ONT) offers a potential alternative using the compact and cost-effective flow cells.
View Article and Find Full Text PDFJ Microbiol Biol Educ
September 2025
University of California Riverside, Riverside, California, USA.
DNA literacy is becoming increasingly essential for navigating healthcare, understanding pandemics, and engaging with biotechnology-yet genomics education remains limited at the secondary level of education. We present a modular, hands-on curriculum designed for high school and early undergraduate students (ages 14-21) that introduces key genomics concepts through an experiment on fermentation, a process that is key to food preservation and medicine. Students follow a complete scientific process: exploring what DNA is and how microbial succession works, analyzing real DNA sequencing data, and writing a formal scientific report.
View Article and Find Full Text PDFAnal Biochem
September 2025
Vegetable and Fruit Improvement Center and Department of Horticultural Sciences Texas A&M University, College Station, TX, 77843-2133, USA; Molecular and Environmental Plant Sciences, Texas A&M University, College Station, TX, 77843-2133, USA. Electronic address:
Whole plasmid sequencing (WPS) using Nanopore long read sequencing has emerged as a cost-effective alternative for dideoxy sequencing methods. De novo sequence assembly for large plasmids, however, are not always successful and may produce large assembly gaps. Here we streamlined a reference-guided alignment of WPS nanopore reads using galaxy platform.
View Article and Find Full Text PDFACS Appl Mater Interfaces
September 2025
School of Material Science and Engineering, Beijing University of Chemical Technology, 15 North Third Ring Road, Chaoyang, Beijing 100029, China.
The construction of perfluoropolyether (PFPE) slippery liquid-infused porous surfaces (SLIPS) on gold coatings is one of the most effective strategies for bestowing anticoagulation and antimicrobial properties on the material. However, the poor chemical affinity between fluorinated porous precursors and gold substrates causes the agglomeration of nanostructures, resulting in uneven nanoporous morphology and accelerating lubricant leakage. Simultaneously, the weak interfacial adhesion between the nanostructures and the substrate may lead to the detachment of nanostructures under blood circulation.
View Article and Find Full Text PDFSmall
September 2025
Faculty of Electrical Engineering, Częstochowa University of Technology, Al. Armii Krajowej 17, Częstochowa, 42-200, Poland.
Bent-core nematic liquid crystals exhibit unique properties, including giant flexoelectricity and polar electro-optic responses, making them ideal for energy conversion and electro-optic applications. When confined in nanopores, they can stabilize chiral nanostructures, enhance polar order, and enable defect-driven switching - offering potential in nanofluidics, sensing, and adaptive optics. The thermotropic ordering of the bent-core dimer CB7CB confined in anodic aluminum oxide (AAO) and silica membranes with precisely engineered cylindrical nanochannels - ranging from just a few nanometers to several hundred nanometers-is examined.
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