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In this commentary, we focused our attention on capillary electrophoresis. It achieves the efficient separation of molecular species by the application of high voltages to samples in solution. Actually, capillary electrophoresis can be performed on microchip devices, based on an automated and miniaturized electrophoresis system, based on lab-on-a-chip technology. By this technology it is possible to separate nucleic acid fragments (DNA or RNA) with respect to sizing accuracy and sizing resolution. Currently, two automated capillary electrophoresis on microchips devices are available: the Agilent 2100 Bioanalyzer and the Experion™ Automated Electrophoresis System. In this study, we evaluated if the CE is able to distinguish the three uridine diphosphate glucuronosyltransferase 1A1 TATA-box genotypes.
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http://dx.doi.org/10.1002/jssc.201400235 | DOI Listing |
Clin Lab
September 2025
Background: Light chain multiple myeloma (LCMM) is a malignant hematological disease characterized by bone marrow infiltration by tumor plasma cells and the secretion of monoclonal free light chains (κ or λ). It is often di-agnosed through hypogammaglobulinemia detected by serum protein electrophoresis, followed by immunotyping showing a monoclonal band in free light chains. However, the structure of monoclonal light chains can sometimes complicate laboratory findings.
View Article and Find Full Text PDFCell Mol Biol (Noisy-le-grand)
September 2025
Doctorado en Genética Humana, Centro Universitario de Ciencias de la Salud. Universidad de Guadalajara, Jalisco, México.
The objective of this study was to evaluate the concentration and integrity index of circulating cell-free DNA (ccf-DNA) as biomarkers for the detection and monitoring of minimal residual disease (MRD) in pediatric patients with B-cell acute lymphoblastic leukemia (B-ALL). Comparison with a validated methodology for the quantification of monoclonal rearrangements of the IGH gene was made. Peripheral blood and bone marrow samples were collected from 10 pediatric patients with B-ALL at diagnosis, remission, and maintenance phases.
View Article and Find Full Text PDFForensic Sci Int Synerg
December 2025
DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines Diliman, Quezon City 1101 Philippines.
Massively parallel sequencing (MPS) has caused a paradigm shift in forensic DNA analysis by enabling simultaneous examination of multiple genetic markers with higher resolution. Despite its growing importance, adoption in the 11 Southeast Asian countries remains limited. This paper reviews MPS implementation in forensic DNA laboratories across the region and discusses key adoption challenges.
View Article and Find Full Text PDFElectrophoresis
September 2025
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, and Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, People's Republic of China.
Foodborne pathogenic bacteria always threaten human health. Flavonoids are commonly used in antibacterial applications. Studying the antibacterial effect of flavonoids on bacteria is significant.
View Article and Find Full Text PDFElectrophoresis
September 2025
Therapeutics Development and Supply-Analytical Development, Janssen Research & Development, LLC, Malvern, Pennsylvania, USA.
Monoclonal antibodies (mAbs) present analytical challenges due to their inherent heterogeneity and susceptibility to post-translational modifications (PTMs) during production and storage. Monitoring of charge heterogeneity profiles by imaged capillary isoelectric focusing (icIEF) has been aided by the use of non-detergent sulfobetaines (NDSBs), particularly NDSB-211, to enhance protein solubility and stability. When used in a quality control laboratory setting, NDSB-211 has shown performance variability over time due to residual manufacturing impurities that impact the capillary isoelectric focusing separation.
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