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The ability to exchange DNA between cells is a molecular process that exists in different species in the domain Archaea. Such horizontal gene transfer events were shown to take place between distant species of archaea and to result in the transfer of large genomic regions. Here we describe recent progress in this field, discuss the potential use of natural gene exchange processes to perform genome shuffling and argue its possible biotechnological applications.
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http://dx.doi.org/10.4161/bioe.22649 | DOI Listing |
NAR Genom Bioinform
September 2025
DNA Repair and Recombination Laboratory, St Vincent's Institute of Medical Research, Fitzroy VIC 3065, Australia.
Meiotic crossovers promote correct chromosome segregation and the shuffling of genetic diversity. However, the measurement of crossovers remains challenging, impeding our ability to decipher the molecular mechanisms that are necessary for their formation and regulation. Here we demonstrate a novel repurposing of the single-nucleus Assay for Transposase Accessible Chromatin with sequencing (snATAC-seq) as a simple and high-throughput method to identify and characterize meiotic crossovers from haploid testis nuclei.
View Article and Find Full Text PDFJ Fungi (Basel)
August 2025
National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China.
, a fungal species of within the Pluteaceae family, is predominantly cultivated in southern China. Polysaccharides, the primary bioactive constituents of , exhibit diverse pharmacological activities. However, current cultivation practices face challenges due to the genetic heterogeneity of strains, leading to inconsistent content and compositional variability of polysaccharides and other functional components.
View Article and Find Full Text PDFEcoSal Plus
August 2025
New England Biolabs Inc., Ipswich, Massachusetts, USA.
Understanding the mechanisms that modulate horizontal genetic exchange in prokaryotes is a key problem in biology. DNA entry is limited by resident host-dependent restriction-modification (RM) systems (HDRM), which are present in most prokaryotic genomes. This review specifically focuses on the biological functions of HDRM, rather than detailed enzyme mechanisms.
View Article and Find Full Text PDFMicrobiol Spectr
September 2025
Université Bordeaux, INRAE, Bordeaux INP, Bordeaux Sciences Agro, UMR1366 Œnologie, F-33140, Villenave d'Ornon, France.
is the predominant lactic acid bacteria species in wine, where it performs the malolactic fermentation, which helps to secure and preserve wine quality. Here, we describe the morphological, biological, and genomic characterization of siphophage Krappator X, a strictly lytic phage that was previously isolated from Merlot wines. Several aspects of the life cycle of the phage were investigated using the sensitive strain IOEBS277 under optimal growth conditions.
View Article and Find Full Text PDFBiosci Rep
August 2025
Department of Medicine, Faculty of Medicine, University of Porto, Porto, Portugal, PT.
The process of selecting indigenous Saccharomyces cerevisiae strains as a starter culture for specific fermentation has several requisites, including the assessment of distinct parameters based on desirable and traditional oenological criteria. In most wineries, commercial Saccharomyces cerevisiae strains are used for wine fermentation. However, it is rare to find indigenous cerevisiae strains used in wine production, even though these isolates are better adapted to specific regions and are often preferred for producing local fruit wines.
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