Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Two reagents have been synthesized for selective labeling of cell surface azidoglycans, an unusually stable version of a dibenzocyclooctyne (TMDIBO) and a third-generation difluorinated cyclooctyne (DIFO3). Both syntheses are efficient with minimal purification, and the dibenzocyclooctyne is stable under basic and acidic conditions. Flow cytometric measurements with azidosugar labeled cancer cells, in which these reagents were linked to the fluorophore Alexa Fluor 647, gave a signal-to-background ratio of up to 35 with TMDIBO as compared to ≈10 for DIFO3 and ≈5 for a phosphine reagent. TMDIBO-based probes should have applications in molecular imaging of cell surface glycans in vivo.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3378185 | PMC |
| http://dx.doi.org/10.1039/C0SC00631A | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Trp-containing Peptides with Therapeutic Potential for Cutibacterium acnes Infection.
Probiotics Antimicrob Proteins
September 2025
School of Life Science, Liaoning Normal University, Dalian, 116081, China.
Cutibacterium acnes (C. acnes, formerly classified as Propionibacterium acnes) is a Gram-positive bacterium that contributes to the development of acne vulgaris, resulting in inflammation and pustule formation on the skin. In this study, we developed and synthesized a series of antimicrobial peptides (AMPs) that are derived from the skin secretion of Rana chensinensis.
View Article and Find Full Text PDFNPY-functionalized niosomes for targeted delivery of margatoxin in breast cancer therapy.
Med Oncol
September 2025
Venom and Biotherapeutics Molecules Laboratory, Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Neuropeptide Y (NPY) and the voltage-gated potassium channel Kv1.3 are closely associated with breast cancer progression and apoptosis regulation, respectively. NPY receptors (NPYRs), which are overexpressed in breast tumors, contribute to tumor growth, migration, and angiogenesis.
View Article and Find Full Text PDFMulti-scale model of neural entrainment by transcranial alternating current stimulation in realistic cortical anatomy.
J Comput Neurosci
September 2025
School of Electrical and Information Engineering, Tianjin University, Tianjin, 300072, China.
Transcranial alternating current stimulation (tACS) enables non-invasive modulation of brain activity, holding promise for cognitive research and clinical applications. However, it remains unclear how the spiking activity of cortical neurons is modulated by specific electric field (E-field) distributions. Here, we use a multi-scale computational framework that integrates an anatomically accurate head model with morphologically realistic neuron models to simulate the responses of layer 5 pyramidal cells (L5 PCs) to the E-fields generated by conventional M1-SO tACS.
View Article and Find Full Text PDFEscherichia coli strain O55 contains two cryptic plasmids that depend on each other to replicate.
Arch Microbiol
September 2025
División de Ciencias Naturales y Exactas, Departamento de Biología, Universidad de Guanajuato, Zip Code 36050, Guanajuato, Mexico.
Plasmids are fundamental to molecular biology and biotechnology, playing a crucial role in bacterial evolution. Some plasmids are linked to complex cellular dynamics, including pathogenicity islands, antibiotic resistance, and gene mobilization. This study reports the isolation and sequencing of two cryptic plasmids with different electrophoretic mobilities from the Escherichia coli clinical isolate O55.
View Article and Find Full Text PDFProto-SLIPS: Slippery Liquid-Infused Surfaces that Release Highly Water-Soluble Agents.
ACS Appl Mater Interfaces
September 2025
Department of Chemistry, University of Wisconsin-Madison, 1101 University Ave., Madison, Wisconsin 53706, United States.
Slippery liquid-infused porous surfaces (or "SLIPS") can prevent bacterial surface fouling, but they do not inherently possess the means to kill bacteria or reduce cell loads in surrounding media. Past reports show that the infused liquids in these materials can be leveraged to load and release antimicrobial agents, but these approaches are generally limited to the use of hydrophobic agents that are soluble in the infused oily phases. Here, we report the design of so-called "proto-SLIPS" that address this limitation and permit the release of highly water-soluble (or oil-insoluble) agents.
View Article and Find Full Text PDF