Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Structures of complete 10-subunit yeast TFIIH and of a nested set of subcomplexes, containing 5, 6, and 7 subunits, have been determined by electron microscopy (EM) and 3D reconstruction. Consistency among all the structures establishes the location of the "minimal core" subunits (Ssl1, Tfb1, Tfb2, Tfb4, and Tfb5), and additional densities can be specifically attributed to Rad3, Ssl2, and the TFIIK trimer. These results can be further interpreted by placement of previous X-ray structures into the additional densities to give a preliminary picture of the RNA polymerase II preinitiation complex. In this picture, the key catalytic components of TFIIH, the Ssl2 ATPase/helicase and the Kin28 protein kinase are in proximity to their targets, downstream promoter DNA and the RNA polymerase C-terminal domain.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277522 | PMC |
| http://dx.doi.org/10.1073/pnas.1105266109 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of a ligand on the asymmetric hydrogenation of cyclic -sulfonyl amines catalyzed by nickel.
Org Biomol Chem
September 2025
School of Chemistry & Environment; Yunnan Key Laboratory of Chiral Functional Substance Research and Application, Yunnan Minzu University, Yuehua Street, Kun-ming 650504, China.
The present study utilizes density functional theory (DFT) to systematically investigate the effect of a ligand on the mechanism of nickel-catalyzed asymmetric hydrogenation of cyclic -sulfonyl imines, employing alcohol protons as the hydrogen source. By comparing the free energies of three catalytic pathways involving various coordinated nickel complexes with different ligands, we identify that the enantio-determining step is the nickel-hydride transfer. Notably, the reaction pathway initiated by the Ni(0) species through oxidative addition of alcohol is determined to be the most favorable.
View Article and Find Full Text PDFThermopower regulation of thermocells electrolyte engineering: progress and prospects.
Chem Commun (Camb)
September 2025
Guangdong Provincial Key Laboratory of New Energy Materials Service Safety, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518055, China.
Thermocells (TECs) represent a promising technology for sustainable low-grade waste heat (<100 °C) harvesting, offering distinct advantages such as scalability, structural versatility, and high thermopower. However, their practical applications are still hindered by low energy conversion efficiency and stability issues. In recent studies, electrolyte engineering has been highlighted as a critical strategy to enhance their thermopower by regulating the solvation structure and redox ion concentration gradient, thereby improving conversion efficiency.
View Article and Find Full Text PDFSingle-Molecule Dual-Anchor Design Enables Extreme-Condition Lithium Metal Batteries Through Solvation Reconstruction and Cathode Polymerization.
Angew Chem Int Ed Engl
September 2025
State Key Laboratory of Chemical Resource Engineering, Beijing Key Laboratory of Electrochemical Process and Technology of Materials, Beijing University of Chemical Technology, Beijing, 10029, P.R. China.
Lithium metal batteries (LMBs) have emerged as the most promising candidate for next-generation high-energy-density energy storage systems. However, their practical implementation is hindered by the inability of conventional carbonate electrolytes to simultaneously stabilize the lithium metal anode and LiNiCoMnO (NCM811) cathode interfaces, particularly under extreme operating conditions. Herein, we present a transformative molecular design using 3,5-difluorophenylboronic acid neopentyl glycol ester (DNE), which uniquely integrates dual interfacial stabilization mechanisms in a single molecule.
View Article and Find Full Text PDFSex-specific association of novel complex inflammatory markers with colorectal polyps: mediating effects of triglyceride-glucose and non-high-density lipoprotein cholesterol.
Lipids Health Dis
September 2025
The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.
Background: The CRP-albumin-lymphocyte (CALLY) index has potential clinical value as a novel marker integrating inflammatory, nutritional and immune status in the development of colorectal polyps. This study examined whether gender factors influence the association between CALLY and colorectal polyps; in addition to elucidating whether metabolic pathways mediate this relationship.
Methods: This is a cross-sectional study including 5409 adult health screening participants who completed colonoscopy.
Age-related changes in cardiolipin profile and functional consequences of altered fatty acid supply.
Biochim Biophys Acta Mol Cell Biol Lipids
September 2025
Department of Anesthesiology and Intensive Care Medicine, Jena University Hospital, Am Klinikum 1, 07747, Jena, Germany; Center for Molecular Biomedicine, Jena University Hospital, Hans-Knöll-Str. 2, 07745, Jena, Germany; Center for Sepsis Control and Care, Jena University Hospital, Am Klinikum 1,
Cardiolipins (CLs) are primarily expressed in the inner mitochondrial membrane where they play essential roles in membrane architecture and mitochondrial functions. CLs have a unique structure characterized by four acyl chains with different stoichiometries such as chain length and degree of saturation. CL composition changes with disease and age, but it is largely unknown how dynamic changes affect mitochondrial function.
View Article and Find Full Text PDF