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http://dx.doi.org/10.1002/chem.201102732 | DOI Listing |
Methods Enzymol
August 2025
Department of Life Sciences, Korea University, Seoul, Republic of Korea. Electronic address:
Formylmethionine (fMet) plays crucial roles across bacterial and eukaryotic systems, contributing to protein translation, degradation, complex formation, stress adaptation, disease progression, and immune response. However, detecting fMet-bearing (fMet-) peptides and proteins has remained challenging due to the lack of effective anti-pan-fMet antibodies. We developed a polyclonal pan-fMet-specific antibody using a single antigen peptide, fMet-Gly-Ser-Gly-Cys pentapeptide, and a mixed antigen peptide, fMet-Xaa-Cys (Xaa, any of the 20 amino acids) tripeptides, as the immunogen.
View Article and Find Full Text PDFNPJ Biofilms Microbiomes
August 2025
Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany.
Peri-implantitis is a severe biofilm-associated infection affecting millions worldwide. This cross-sectional study aimed to identify taxonomic and functional biomarkers that reliably indicate peri-implantitis by utilizing paired data from full length 16S rRNA gene amplicon sequencing (full-16S) and metatranscriptomics (RNAseq) in 48 biofilm samples from 32 patients. Both full-16S and RNAseq analyses revealed significant differences between healthy and peri-implantitis samples, with a shift toward anaerobic Gram-negative bacteria in peri-implantitis.
View Article and Find Full Text PDFbioRxiv
August 2025
Center for Discovery and Innovation in Parasitic Diseases, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA.
The 20S proteasome (Pf20S) is a promising antimalarial target. Therapeutic development has previously relied on native purifications of Pf20S, which is challenging and has limited the scope of previous efforts. Here, we report an effective recombinant Pf20S platform to facilitate drug discovery.
View Article and Find Full Text PDFLuminescence
August 2025
Precise Synthesis and Function Development Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Nanchong, China.
Herein, a new large Stokes shift fluorescent probe DGCA based on dansyl group modified tripeptide molecule (Gly-Cys-Ala-NH) was successfully synthesized. DGCA acts as a promising probe for simultaneous detection of Ag and Cu based on different response modes, which displayed fluorescence enhancement by Ag and fluorescence quenching by Cu in 100% aqueous solution. In addition, DGCA exhibited high sensitivity recognition of Ag and Cu, with the limits of detections (LODs) being 7.
View Article and Find Full Text PDFJ Pept Sci
September 2025
Suzhou Chien-Shiung Institute of Technology, Suzhou, Jiangsu, China.
Nrf2-Keap1 is an important defense system against oxidative stress damage and enhances the body's antioxidant capacity. Targeting the Keap1-Nrf2 signaling pathway and activating Nrf2 has become an effective strategy for treating oxidative stress and related diseases. In this study, virtual digestion, ADMET prediction, and molecular docking were used to screen antioxidant peptide from silkworm pupa protein.
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