Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Pathologic rheumatoid factor (RF) levels are hallmarks of several human diseases. Production of monoclonal RF in vitro is essential for studies of the antigenic specificities of RF, as well as for a dissection of the mechanisms of aberrant RF+ B cell activation. We have expanded upon previous methods to develop a flow cytometry-based method to efficiently clone monoclonal antibodies (mAbs) from humans with expansions of RF-like, immunoglobulin heavy chain variable region (IgVH) 1-69 gene segment-containing B cells. The cloned variable regions are expressed as IgM and produced during culture at concentrations between 5 and 20 μg/ml. Using this system, we show that clonal Igs from patients with HCV-related mixed cryoglobulinemia, when expressed as IgM, have RF activity. We anticipate that this system will be useful for the cloning and expression of mAbs partially encoded by VH1-69 and for determination of the reactivity patterns of polyspecific, low-affinity IgMs of human pathogenic importance.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003765 | PMC |
| http://dx.doi.org/10.1016/j.jim.2010.09.022 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Intracellular lymphocyte protein biomarkers for early radiological triage in the human population.
PLoS One
September 2025
Center for Radiological Research, Columbia University Irving Medical Center, New York, New York, United States of America.
In the event of a large-scale radiological or nuclear emergency, a rapid, high-throughput screening tool will be essential for efficient triage of potentially exposed individuals, optimizing scarce medical resources and ensuring timely care. The objective of this work was to characterize the effects of age and sex on two intracellular lymphocyte protein biomarkers, BAX and p53, for early radiation exposure classification in the human population, using an imaging flow cytometry-based platform for rapid biomarker quantification in whole blood samples. Peripheral blood samples from male and female donors, across three adult age groups (young adult, middle-aged, senior) and a juvenile cohort, were X-irradiated (0-5 Gy), and biomarker expression was quantified at two- and three-days post-exposure.
View Article and Find Full Text PDFClathrin-Mediated Endocytosis for Enhanced Apoptosis Induction and Cellular Imaging in Triple-Negative Breast Cancer by Casein-Protected Blue-Emissive Carbon Dots.
ACS Appl Bio Mater
September 2025
Chemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
The development of multifunctional nanoplatforms capable of drug delivery and real-time cellular imaging remains a key challenge in cancer theranostics. Herein, we report the development of a casein-protected maleic acid-derived nitrogen-doped carbon dot-based luminescent nanoplatform (MNCD@Cas NPs) for efficient delivery of the anticancer drug doxorubicin hydrochloride (DOX) to triple-negative breast cancer cells. Synthesized via a facile two-step method, the MNCD@Cas NPs exhibit bright blue fluorescence (λ = 390 nm), high water dispersibility, excellent colloidal stability, and substantial DOX loading capacity (∼84%) driven by electrostatic interactions.
View Article and Find Full Text PDFRapid Diagnosis and Subtyping of Hermansky-Pudlak Syndrome With Flow Cytometry Analysis.
Pigment Cell Melanoma Res
September 2025
Department of Dermatology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
The diagnostic approaches for Hermansky-Pudlak Syndrome (HPS) include genetic sequencing, immunoblotting, electron microscopy (EM), and flow cytometry with mepacrine staining. However, these methods are often impractical for routine clinical use due to high cost, technical complexity, and limited availability. In this study, we evaluated dense granules (DGs) function in HPS mouse models using flow cytometry with mepacrine and FluoZin-3 staining.
View Article and Find Full Text PDFHIV-1 manipulates CD96 on CD4 T cells to subvert antiviral immunity.
Sci Adv
September 2025
Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital Tübingen, Tübingen, Germany.
HIV-1 evades immune responses by modulating plasma membrane receptors. Using a flow cytometry-based screening, we profiled 332 surface receptors on HIV-1-infected primary CD4 T cells and identified 23 down-regulated receptors, including known targets such as CD4, MHCI, CCR7, and CD62L. CD96, an inhibitory natural killer (NK) cell receptor poorly studied in human CD4 T cells, was markedly down-regulated.
View Article and Find Full Text PDFHarmonized Immune Recovery Monitoring after HCT: Evidence & Practical Guidance from the Westhafen Intercontinental Group.
Blood Adv
September 2025
Memorial Sloan Kettering Cancer Center, New York, New York, United States.
Allogeneic hematopoietic cell transplantation (allo-HCT) is a curative option for patients with high-risk malignancies and non-malignant disorders. Long-term survival depends on robust immune reconstitution (IR), which governs overall immune homeostasis and risks of infection, graft-versus-host disease, and relapse. However, despite its centrality to post-transplant outcomes, IR is not consistently monitored across transplant centers, limiting ability to generate meaningful, comparable, and translatable data.
View Article and Find Full Text PDF